Abstract | BACKGROUND AND PURPOSE: Common etiologies of acute enterocolitis in childhood include the intracellular pathogens Salmonella and rotavirus, along with extracellular pathogens. In order to elucidate differentiating immunologic parameters in patients with acute gastroenteritis of different etiologies, we investigated interferon (IFN)-gamma, interleukin (IL)-12, and T-bet of T-helper type 1 subsets, and IL-4 and GATA-3 of T-helper type 2 subsets. METHODS: From June 1, 2003 to December 31, 2003, 32 patients with acute gastroenteritis were enrolled. Sequential heparinized blood samples were obtained on the day of presentation (day 1) and on day 3 of hospitalization. Using reverse transcriptase-polymerase chain reaction, the mean ratios of IFN-gamma, T-bet and IL-12 mRNA levels relative to beta-actin were determined. RESULTS: CONCLUSIONS:
|
Authors | An-Yi Lin, Ching-Yuang Lin, Ching-Tung Chen, Woan-Ling Chen |
Journal | Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
(J Microbiol Immunol Infect)
Vol. 41
Issue 3
Pg. 265-71
(Jun 2008)
ISSN: 1684-1182 [Print] England |
PMID | 18629423
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Adolescent
- Child
- Child, Preschool
- Cytokines
(biosynthesis)
- Female
- Gastroenteritis
(immunology)
- Gene Expression Profiling
- Humans
- Infant
- Infant, Newborn
- Leukocytes, Mononuclear
(immunology)
- Male
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Rotavirus
(immunology)
- Rotavirus Infections
(immunology)
- Salmonella
(immunology)
- Salmonella Infections
(immunology)
- Time Factors
|