Determination of local anaesthetics in body fluids by gas chromatography with surface ionization detection.

Abstract

Ten local anaesthetics were tested for their detection by gas chromatography (GC)-surface ionization detection (SID). Lidocaine, mepivacaine and bupivacaine were detected with the highest sensitivity; their detection limit was 5-10 pg in an injected volume. The sensitivity of other drugs, such as procaine, dibucaine tetracaine and oxybuprocaine, was an order of magnitude lower than that of the above three local anaesthetics. A detailed procedure for isolation of local anaesthetics from human whole blood and cerebrospinal fluid (CSF) by the use of Sep-Pak C18 cartridges, before the GC-SID, is also presented. The recovery of lidocaine, mepivacaine and bupivacaine, which had been added to 1 ml of whole blood or CSF, was close to 100%.

• Authors: H Hattori, S Yamamoto, T Yamada, O Suzuki
• Journal: Journal of chromatography (J Chromatogr) Vol. 564 Issue 1 Pg. 278-82 (Mar 08 1991) Netherlands
• PMID: 1860922 (Publication Type: Journal Article)

Chemical References

• Anesthetics, Local
• Lidocaine
• Mepivacaine
• Bupivacaine

Topics

• Anesthetics, Local (analysis, blood, cerebrospinal fluid)
• Body Fluids (chemistry)
• Bupivacaine (blood, cerebrospinal fluid)
• Chromatography, Gas (methods)
• Humans
• Lidocaine (blood, cerebrospinal fluid)
• Mepivacaine (blood, cerebrospinal fluid)