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Systemic and mucosal immunization with Candida albicans hsp90 elicits hsp90-specific humoral response in vaginal mucosa which is further enhanced during experimental vaginal candidiasis.

Abstract
The Candida albicans heat shock protein 90 kDa (hsp90-CA) is an important target for protective antibodies in disseminated candidiasis of experimental mice and humans. Hsp90-CA is present in the cell wall of Candida pseudohyphae or hyphae--typical pathogenic morphotypes in both mucosal and systemic Candida infections. However, the potential protective effects of hsp90-CA-specific antibodies in vaginal candidiasis has not yet been reported. In the present study we used various vaccine formulations (recombinant hsp90-CA protein and hsp90-CA-encoding DNA vaccine) and routes of administration (intradermal, intranasal, and intravenous) to induce both hsp90-CA-specific systemic and vaginal mucosa immune responses in experimental BALB/c mice. The results showed that intradermal recombinant hsp90-CA protein priming, followed by intranasal or intradermal recombinant hsp90-CA protein boosting induced significant increases in both serum and vaginal hsp90-CA-specific IgG and IgA antibodies compared to the control group, as well as enhanced hsp90-CA-specific splenocyte responses in vitro. In the intradermally boosted group, subsequent experimental vaginal Candida infection induced additional increases in the hsp90-CA specific IgG isotype, suggesting that Candida has the ability to induce a local hsp90-specific antibody (IgG) response during vulvovaginal candidiasis. Further work is required to elucidate the importance of immunity to highly conserved antigens during infection of the human female reproductive tract where a balance between immunity to and tolerance for commonly antigens such as hsp90 is necessary for the maintenance of fertility.
AuthorsMilan Raska, Jana Belakova, Milada Horynova, Michal Krupka, Jiri Novotny, Martina Sebestova, Evzen Weigl
JournalMedical mycology (Med Mycol) Vol. 46 Issue 5 Pg. 411-20 (Aug 2008) ISSN: 1369-3786 [Print] England
PMID18608941 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Fungal
  • Fungal Proteins
  • Fungal Vaccines
  • HSP90 Heat-Shock Proteins
  • Vaccines, DNA
  • Vaccines, Synthetic
Topics
  • Administration, Intranasal
  • Animals
  • Antibodies, Fungal (analysis, blood)
  • Antibody Formation
  • Candida albicans (immunology)
  • Candidiasis, Vulvovaginal (immunology)
  • Cell Proliferation
  • Female
  • Fungal Proteins (immunology)
  • Fungal Vaccines (administration & dosage, immunology)
  • HSP90 Heat-Shock Proteins (immunology)
  • Immunization, Secondary
  • Injections, Intradermal
  • Injections, Intravenous
  • Lymphocytes (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Spleen (immunology)
  • Vaccines, DNA (administration & dosage, immunology)
  • Vaccines, Synthetic (administration & dosage, immunology)
  • Vagina (immunology, microbiology)

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