Abstract | OBJECTIVES: To seek an interrelationship, if any, between oxidant stress and neurochemical changes in various rat brain regions after arsenic exposure. MATERIALS AND METHODS: This study was carried out at the Department of Biochemistry, Al Arab Medical University, Benghazi, Libya. Seventy five male Spraque-Dawley rats were divided into three groups: CONTROL GROUP: Rats were administered 2 ml of normal saline solution/kg body weight (b.wt.) daily for 20 days by intraperitoneal (i.p.) route. ARSENIC-TREATED GROUP: Rats received elemental arsenic (as sodium arsenate) 2.0 mg/kg b.wt. daily for 20 days by i.p. route. RECOVERY GROUP: Rats received 2.0 mg/kg b.wt. elemental arsenic daily for 20 days by i.p. route and were allowed to recover for 20 days. Rats were sacrificed and brains were dissected into cerebral cortex, corpus striatum, cerebellum and brain stem. Tissue homogenized in respective mediums. And were analyzed for lipid classes, oxidative stress, concentration of proteins, glutathione and ascorbic acid by utilizing standard colorimetric procedures. RESULTS: CONCLUSION: These results suggested that arsenic-initiated oxidant stress by increasing lipid peroxidation. The losses of lipid classes, ascorbic acid and glutathione may be attributed to peroxidative damage and binding of arsenic with sulfhydryl groups of enzymes. Recovery of animals showed reversibility in most of studied parameters, but gangliosides and cerebrosides over shooted. And speculated " Sphingolipidosis". It is then likely that repeated exposures of humans to arsenic may result in hampering of cell signalling, apoptosis and mutagenesis.
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Authors | S S Haider, M S Najar |
Journal | Kathmandu University medical journal (KUMJ)
(Kathmandu Univ Med J (KUMJ))
2008 Jan-Mar
Vol. 6
Issue 1
Pg. 60-9
ISSN: 1812-2078 [Electronic] Nepal |
PMID | 18604117
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Arsenates
- Sulfhydryl Compounds
- sodium arsenate
- Glutathione
- Ascorbic Acid
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Topics |
- Animals
- Antioxidants
(metabolism)
- Arsenates
(toxicity)
- Ascorbic Acid
(metabolism)
- Brain
(metabolism)
- Chromatography, Thin Layer
- Glutathione
(metabolism)
- Lipid Peroxidation
- Male
- Oxidative Stress
- Protein Biosynthesis
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Sphingolipidoses
(chemically induced)
- Sulfhydryl Compounds
(metabolism)
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