Eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone), an extract from Artemisia asiatica Nakai, is a
flavonoid of pharmacologically active ingredients.
Eupatilin is known to possess anti-
cancer, anti-inflammatory, and anti-oxidative activity. Recently,
eupatilin has been reported to be effective in producing gastric mucosal as an anti-
gastritis agents. However, the mechanism of protective action is still unknown. We studied cytoprotective actions of
eupatilin on H(2)O(2)-induced cell death and its possible mechanisms of action in human gastric (AGS) cells.
Eupatilin dose-dependently inhibited H(2)O(2)-induced apoptosis as indicated by co-staining with
Annexin V and
propidium iodide.
Hydrogen peroxide provoked phosphorylation of extracellular regulated
kinase (ERK) and c-Jun NH(2)-terminal
kinase (JNK), and activation of
nuclear factor-kappaB (
NF-kappaB). On the contrary,
eupatilin decreased H(2)O(2)-induced activation of ERK, JNK and
NF-kappaB. In addition, treatment of specific inhibitors for ERK, JNK, and
NF-kappaB attenuated H(2)O(2)-induced apoptosis. Co-treatment of inhibitors and
eupatilin was more effective in decreasing H(2)O(2)-induced apoptosis. Taken together, we suggest that
eupatilin inhibits H(2)O(2)-induced apoptosis through the inhibition ERK, JNK, and
NF-kappaB.