Abstract |
Bisacurone, one of the active compounds of the traditionally used indigenous herb Curcuma longa Linne (Zingiberaceae), has anti-oxidant, anti-inflammatory, and anti-metastatic activities. We studied how the level of vascular cell adhesion molecule-1 (VCAM-1), one of the key molecules in the development of atherosclerosis as well as carcinogenesis and metastasis, might be affected by bisacurone in tumor necrosis factor-alpha ( TNF-alpha)-activated human umbilical vein endothelial cells (HUVECs). Bisacurone dose-dependently inhibited TNF-alpha-mediated expression of VCAM-1. It showed significant suppressive effect on ROS generation in response to TNF-alpha stimulation and it blocked nuclear factor-kappa B ( NF-kappaB) p65 translocation into the nucleus and phosphorylation of inhibitory factor kappaBalpha ( IkappaBalpha). It also inhibited phosphorylation of Akt and PKC, which are upstream in the regulation of VCAM-1 by TNF-alpha. Furthermore, bisacurone decreased U937 monocyte and human oral cancer cell (Hep-2, QLL-I, SCC-15) adhesion to HUVECs stimulated by TNF-alpha, suggesting that it may inhibit the binding of these cells by regulating the expression of critical adhesion molecules by TNF-alpha. Thus, bisacurone may be beneficial in the treatment of inflammatory diseases, such as atherosclerosis, where inflammatory monocytes are involved in their pathology, and, moreover, in the development of tumors.
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Authors | Dong-Il Sun, Irina Tsoy Nizamutdinova, Young Min Kim, Xing Fu Cai, Jung Joon Lee, Sam Sik Kang, Yeong Shik Kim, Ki Mun Kang, Gyu Young Chai, Ki Churl Chang, Hye Jung Kim |
Journal | International immunopharmacology
(Int Immunopharmacol)
Vol. 8
Issue 9
Pg. 1272-81
(Sep 2008)
ISSN: 1567-5769 [Print] Netherlands |
PMID | 18602074
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Cyclohexanols
- Oxidants
- Plant Extracts
- Sesquiterpenes
- Tumor Necrosis Factor-alpha
- Vascular Cell Adhesion Molecule-1
- bisacurone
- Luciferases
- Oncogene Protein v-akt
- Protein Kinase C
- Extracellular Signal-Regulated MAP Kinases
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Topics |
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Blotting, Western
- Cell Adhesion
(drug effects)
- Cells, Cultured
- Cyclohexanols
(pharmacology)
- Down-Regulation
(drug effects)
- Endothelial Cells
(metabolism, pathology)
- Extracellular Signal-Regulated MAP Kinases
(biosynthesis, genetics)
- Genes, Reporter
- Humans
- Inflammation
(metabolism, pathology)
- Luciferases
(genetics)
- Monocytes
(drug effects)
- Oncogene Protein v-akt
(biosynthesis, genetics)
- Oxidants
(metabolism)
- Plant Extracts
(chemistry, pharmacology)
- Plasmids
(genetics)
- Protein Kinase C
(biosynthesis, genetics)
- Sesquiterpenes
(pharmacology)
- Transfection
- Tumor Necrosis Factor-alpha
(biosynthesis, pharmacology)
- Vascular Cell Adhesion Molecule-1
(biosynthesis)
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