Treatment with SI000413, a new herbal formula, ameliorates murine collagen-induced arthritis.

Abstract

We tested the effects of SI000413, a new formula, consisting of Pyrolae herba and Trachelospermi caulis, on type II collagen-induced arthritis (CIA). CIA was induced in DBA/1J mice by immunization with bovine type II collagen (CII) on days 1 and 21. SI000413 was orally administered 3 times per week throughout the experiment and indomethacin was served as a positive control. Clinical scores, the count of arthritic legs, levels of interleukin 6 (IL-6) and anti-CII antibody, and lymphocyte subsets in blood were examined. SI000413 suppressed CIA development in a dose dependent manner and reduced the incidence of arthritic legs in mice. Histological analysis showed administration of SI000413 reduced inflammatory signs and cartilage destruction. Serum levels of IL-6 and anti-CII antibody were significantly decreased in SI000413-treated mice and the percentages of CD4 T cell, CD8 T cell and B cell in blood were restored to normal levels. In conclusion, we demonstrate that SI000413 ameliorates CIA both clinically and histologically and inhibits the production of anti-CII antibody and pro-inflammatory cytokine in the CIA mouse. These findings suggest that SI000413 is a potential new therapeutic herbal formula for the treatment of RA.

• Authors: Jee-Hun Park, Jeong-Min Lee, Se-Na Kim, Seung-Ha Lee, Sung-Hoon Jun, Jae-Hoon You, Kyoo-Seok Ahn, Hee Kang
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 31 Issue 7 Pg. 1337-42 (Jul 2008) ISSN: 0918-6158 [Print] Japan
• PMID: 18591771 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antirheumatic Agents
• Autoantibodies
• Collagen Type II
• Cytokines
• Furans
• Glucosides
• Interleukin-6
• Plant Extracts
• Plant Preparations
• SI 000413
• arctiin

Topics

• Animals
• Antirheumatic Agents (chemistry, pharmacology)
• Arthritis, Rheumatoid (chemically induced, pathology, prevention & control)
• Autoantibodies (analysis, biosynthesis)
• CD4-Positive T-Lymphocytes (drug effects)
• CD8-Positive T-Lymphocytes (drug effects)
• Cartilage (pathology)
• Chromatography, High Pressure Liquid
• Collagen Type II (immunology)
• Cytokines (metabolism)
• Dose-Response Relationship, Drug
• Flow Cytometry
• Furans (analysis, isolation & purification)
• Glucosides (analysis, isolation & purification)
• Interleukin-6 (analysis, biosynthesis)
• Joints (pathology)
• Lymphocyte Subsets (immunology)
• Male
• Mice
• Mice, Inbred DBA
• Plant Extracts (chemistry, pharmacology)
• Plant Preparations (chemistry, pharmacology)