[Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats].

Abstract

OBJECTIVE:

The present study investigated the inhibitory effects of Chinese herb component sinapine on activity of acetylcholinesterase (AChE) in cerebral homogenate and blood serum of rats.

METHOD:

AChE was prepared from cerebral homogenate and blood serum of rats, respectively. Acetylcholinesterase activity assay kit and Chromatometry were used to detect the AChE activity.

RESULT:

Sinapine significantly inhibited AChE activity in vitro, with more effective on cerebral homogenate (IC50 3.66 micromol x L(-1)) than blood serum (IC50 22.1 micromol x L(-1)).

CONCLUSION:

Sinapine could significantly inhibit the cerebral AChE activity and may be a promising drug used for prevention and cure of Alzheimer's disease as a cholinesterase inhibitor.

• Authors: Ling He, Hai-Tao Li, Sheng-Wei Guo, Li-Fang Liu, Jia-Bin Qiu, Fu Li, Bao-Chang Cai
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 33 Issue 7 Pg. 813-5 (Apr 2008) ISSN: 1001-5302 [Print] China
• PMID: 18589789 (Publication Type: English Abstract, Journal Article)

Chemical References

• Cholinesterase Inhibitors
• sinapine
• Acetylcholinesterase
• Choline

Topics

• Acetylcholinesterase (blood, metabolism)
• Alzheimer Disease (drug therapy, prevention & control)
• Animals
• Brain (cytology, enzymology)
• Choline (analogs & derivatives, pharmacology, therapeutic use)
• Cholinesterase Inhibitors (pharmacology, therapeutic use)
• Rats