[Inducing differentiation effect on cells of serum from mice treated with Yang He decoction].

Abstract

OBJECTIVE:

To observe inducing differention effect on cells of serum from mice treated with Yang He decoction (SMY).

METHODS:

The viability of B16 cells was tested by lactate dehydrogenase (LDH) release assay and sulforhordamine B (SRB) assay. The differentiation of 3T3-L1 preadipocyte, HL-60, K562 and B16 cells were assessed by microscopy and Oil red O staining, NBT reduction and phagocytosis, haemoglobin (Hb) content and melanin content, respectively.

RESULTS:

SMY could obviously inhibit growth of B16 cells without cytotoxicity, and promote Oil red O staining in 3T3-L1 preadipocyte, enhance the NBT reduction ability and the phagocytosis of horse radish peroxidase in HL-60 cells, increase Hb content in K562 cells, and decrease melanin content in B16 cells.

CONCLUSION:

SMY is able to induce functional differentiation and maturation of 3T3-L1 preadipocyte and tumor cells, which implies the major mechanism of Yang He decoction in antitumor.

• Authors: Gang-jun Du, Hai-hong Lin, Song-qiang Xie
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 31 Issue 1 Pg. 82-5 (Jan 2008) ISSN: 1001-4454 [Print] China
• PMID: 18589756 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Culture Media
• Drug Combinations
• Drugs, Chinese Herbal
• yanghe

Topics

• 3T3-L1 Cells
• Animals
• Antineoplastic Agents, Phytogenic (pharmacology)
• Cell Differentiation (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Culture Media (pharmacology)
• Dose-Response Relationship, Drug
• Drug Combinations
• Drugs, Chinese Herbal (administration & dosage, chemistry, pharmacology)
• HL-60 Cells
• Humans
• K562 Cells
• Male
• Mice
• Phagocytosis (drug effects)
• Plants, Medicinal (chemistry)
• Serum