[Inducing differentiation effect on cells of serum from mice treated with Yang He decoction].

To observe inducing differention effect on cells of serum from mice treated with Yang He decoction (SMY).
The viability of B16 cells was tested by lactate dehydrogenase (LDH) release assay and sulforhordamine B (SRB) assay. The differentiation of 3T3-L1 preadipocyte, HL-60, K562 and B16 cells were assessed by microscopy and Oil red O staining, NBT reduction and phagocytosis, haemoglobin (Hb) content and melanin content, respectively.
SMY could obviously inhibit growth of B16 cells without cytotoxicity, and promote Oil red O staining in 3T3-L1 preadipocyte, enhance the NBT reduction ability and the phagocytosis of horse radish peroxidase in HL-60 cells, increase Hb content in K562 cells, and decrease melanin content in B16 cells.
SMY is able to induce functional differentiation and maturation of 3T3-L1 preadipocyte and tumor cells, which implies the major mechanism of Yang He decoction in antitumor.
AuthorsGang-jun Du, Hai-hong Lin, Song-qiang Xie
JournalZhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 31 Issue 1 Pg. 82-5 (Jan 2008) ISSN: 1001-4454 [Print] China
PMID18589756 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Culture Media
  • Drug Combinations
  • Drugs, Chinese Herbal
  • yanghe
  • 3T3-L1 Cells
  • Animals
  • Antineoplastic Agents, Phytogenic (pharmacology)
  • Cell Differentiation (drug effects)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Culture Media (pharmacology)
  • Dose-Response Relationship, Drug
  • Drug Combinations
  • Drugs, Chinese Herbal (administration & dosage, chemistry, pharmacology)
  • HL-60 Cells
  • Humans
  • K562 Cells
  • Male
  • Mice
  • Phagocytosis (drug effects)
  • Plants, Medicinal (chemistry)
  • Serum

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: