Abstract | BACKGROUND: METHODS: Alteration of structure and immune-reactivity of native and pasteurized proteins was assessed by gel filtration and ELISA. Uptake of these proteins was compared in vitro and in vivo. The biological effect was assessed by orally sensitizing C3H/HeJ mice with milk proteins followed by a graded oral challenge. Required dose to induce anaphylaxis, symptoms and mean body temperature was recorded. Antigen-specific antibodies and cytokine production by splenocytes were analyzed. RESULTS: CONCLUSIONS: These results indicate that triggering of an anaphylactic response requires two phases (1) sensitization by aggregates through Peyer's patches and (2) efficient transfer of soluble protein across the epithelial barrier. As the majority of common food allergens tend to form aggregates, this may be of clinical importance.
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Authors | F Roth-Walter, M C Berin, P Arnaboldi, C R Escalante, S Dahan, J Rauch, E Jensen-Jarolim, L Mayer |
Journal | Allergy
(Allergy)
Vol. 63
Issue 7
Pg. 882-90
(Jul 2008)
ISSN: 1398-9995 [Electronic] Denmark |
PMID | 18588554
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Allergens
- Caseins
- Cytokines
- Lactoglobulins
- Lactalbumin
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Topics |
- Administration, Oral
- Allergens
(immunology)
- Anaphylaxis
(immunology)
- Animals
- Antibody Formation
- Body Temperature
- Caseins
(immunology)
- Cytokines
(immunology)
- Dose-Response Relationship, Immunologic
- Enterocytes
(immunology)
- Female
- Food Handling
(methods)
- Hot Temperature
- Lactalbumin
(immunology, metabolism)
- Lactoglobulins
(immunology)
- Mice
- Mice, Inbred C3H
- Milk Hypersensitivity
(immunology)
- Peyer's Patches
(immunology)
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