Dehydroalanine and lysinoalanine in thermolyzed casein do not promote colon cancer in the rat.

Thermolysis of proteins produces xenobiotic amino-acids such as the potentially toxic lysinoalanine, and the alkylating agent, dehydroalanine, which have been considered possible health hazards. We observed that thermolyzed casein promoted aberrant crypt foci (ACF) and colon cancer growth in rats initiated with azoxymethane and speculated that promotion might be due to the formation of these compounds. To test this notion we first measured the concentration of the modified amino acids as a function of thermolysis time. The concentration of dehydroalanine in the casein paralleled the degree of promotion, that of lysinoalanine did not. We then tested diets containing foods with high levels of dehydroalanine (thermolyzed sodium-caseinate, cooked Swiss cheese) for their effect on ACF promotion. They decreased the number and/or size of ACF significantly, indicating that dehydroalanine did not promote, but protected rats against colon carcinogenesis. These results do not support the notion that lysinoalanine or dehydroalanine are a hazard with respect to colon carcinogenicity.
AuthorsDenis E Corpet, Sylviane Taché, Michael C Archer, W Robert Bruce
JournalFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 46 Issue 9 Pg. 3037-42 (Sep 2008) ISSN: 0278-6915 [Print] England
PMID18585427 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Caseins
  • Lysinoalanine
  • dehydroalanine
  • Azoxymethane
  • Alanine
  • Alanine (analogs & derivatives, analysis, metabolism, toxicity)
  • Animals
  • Azoxymethane (toxicity)
  • Carcinogenicity Tests
  • Caseins (chemistry, toxicity)
  • Cheese (analysis)
  • Colonic Neoplasms (chemically induced, pathology)
  • Diet
  • Feces (chemistry)
  • Female
  • Hot Temperature
  • Intestinal Mucosa (pathology)
  • Lysinoalanine (analysis, metabolism, toxicity)
  • Rats
  • Rats, Inbred F344

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