Abstract |
Toxocariasis is a worldwide zoonotic disease caused by the ascarid nematode Toxocara canis. The most common method available for serodiagnosis of toxocariasis is an enzyme-linked immunosorbent assay (ELISA) test using Toxocara excretory-secretory antigen ( TES). The present study describes the development of IgG-ELISA based on antiserum prepared against the recombinant arginine kinase of Toxocara canis. Antiserum was prepared against the purified recombinant arginine kinase (AK) using 6-week-old female Japanese white rabbits. Serum samples were collected from experimentally infected BALB/c and C57BL/6 mice at different time periods. The IgG-ELISA was performed using serum samples from mice (infected/uninfected) and TES antigen with antiserum prepared against the recombinant-AK. The optical density (OD450) was measured at 450 nm using a micro-plate ELISA reader. There were significant differences (P<0.01) in the absorbance between infected and control serum samples. Further, we obtained 100% sensitivity for the serum samples from T. canis-infected mice. Therefore, it is suggested that the recombinant-AK based IgG-ELISA could be applied for immunodiagnosis of human toxocariasis. However, it is necessary to evaluate the specificity of this recombinant antigen with similar geohelminth infections.
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Authors | Susiji Wickramasinghe, Lalani Yatawara, Mitsuru Nagataki, Misa Takamoto, Yoshiya Watanabe, R P V J Rajapakse, Kouji Uda, Tomohiko Suzuki, Takeshi Agatsuma |
Journal | Parasitology research
(Parasitol Res)
Vol. 103
Issue 4
Pg. 853-8
(Sep 2008)
ISSN: 0932-0113 [Print] Germany |
PMID | 18575890
(Publication Type: Evaluation Study, Journal Article)
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Chemical References |
- Antibodies, Helminth
- Antigens, Helminth
- Immunoglobulin G
- Recombinant Proteins
- Arginine Kinase
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Topics |
- Animals
- Antibodies, Helminth
(isolation & purification)
- Antigens, Helminth
(blood, genetics)
- Arginine Kinase
(blood, genetics)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Humans
- Immunoglobulin G
(isolation & purification)
- Larva Migrans, Visceral
(diagnosis)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Rabbits
- Recombinant Proteins
(genetics, immunology)
- Sensitivity and Specificity
- Serologic Tests
- Toxocara canis
(immunology)
- Toxocariasis
(diagnosis)
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