Abstract | BACKGROUND & OBJECTIVE: METHODS: The methylation, mRNA and protein expression of APC gene were detected by methylation-specific polymerase chain reaction (PCR), reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry in 30 specimens of normal proliferative endometrium, 30 specimens of atypical hyperplastic endometrium and 60 specimens of endometrioid adenocarcinoma. RESULTS: The methylation rate of APC gene was significantly higher, the positive rates of APC mRNA and protein were significantly lower in endometrioid adenocarcinoma than in atypical hyperplastic endometrium and normal proliferative endometrium (65.0% vs. 33.3% and 23.3%, 33.3% vs. 63.3% and 73.3%, 30.0% vs. 50.0% and 66.7%,P<0.05). There was no significant difference between atypical hyperplastic endometrium and normal endometrium (P>0.05). APC methylation was positively correlated to APC mRNA expression. CONCLUSION: The expression and DNA methylation of APC gene are certainly related with the occurrence and development of endometrioid adenocarcinoma.
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Authors | Bing Qian, Pei-Qi Ke, Li Wang, Wen-Ji Liu, Mei-Xiang Li |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 27
Issue 6
Pg. 585-9
(Jun 2008)
China |
PMID | 18570730
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adenomatous Polyposis Coli Protein
- RNA, Messenger
|
Topics |
- Adenomatous Polyposis Coli Protein
(analysis, genetics)
- Carcinoma, Endometrioid
(genetics)
- DNA Methylation
- Endometrial Neoplasms
(genetics)
- Female
- Humans
- RNA, Messenger
(analysis)
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