Comparison of PanBio dengue IgM ELISA assay with pentax dengue IgM particle agglutination assay to evaluate factors affecting false positive results.
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| Abstract |
The objectives of this study were to conduct a further evaluation of performance characteristics (sensitivity/specificity, predictive values, cross-reactivity) of PanBio Dengue IgM (IgM-EIA test), particularly during non-epidemic periods in New Caledonia, and (ii) to evaluate an alternative test, Pentax Dengue IgM-Particle Agglutination (PA-lgM) test. A total of 1,808 samples were first tested with the IgM-EIA test and reactive specimens were then re-tested with IgM-PA test. Sensitivity and specificity were measured on a prospective mode from 2005 and 2006. Other etiologies were also investigated to confirm the non-specific reactive results. One hundred fifty-three samples were initially reactive with IgM-EIA test. Of these, 147 were classified as non specific and only 16 were reactive with the particle agglutination test (89.1% reduction of this interference). The specificity and positive predictive value of the ELISA test was 91.8% and 5.8%, respectively. The extrapolated specificity and positive predictive value for the particle agglutination test was 99.1% and 33.3%, respectively. Hepatitis A was identified as a major source of false positive, followed by rheumatoid factor and leptospirosis. Sensitivity of both tests was100% on samples taken from the fifth day of the disease.
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| Authors | Alain Berlioz-Arthaud, Albert Gurusamy |
| Journal | The Southeast Asian journal of tropical medicine and public health (Southeast Asian J Trop Med Public Health) Vol. 39 Issue 1 Pg. 55-61 (Jan 2008) ISSN: 0125-1562 [Print] Thailand |
| PMID | 18567444 (Publication Type: Comparative Study, Evaluation Study, Journal Article) |
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