Characterization and regulation of the expression of scyllatoxin (Leiurotoxin I) receptors in the human neuroblastoma cell line NB-OK 1.

Abstract

125I-[Tyr2]scyllatoxin allowed to label a single class of high-affinity receptors in membranes from the human neuroblastoma cell line NB-OK 1. The Kd of these receptors was 60 pM for scyllatoxin (Leiurotoxin I) and 20 pM for apamin and the Bmax was low (3.8 fmol/mg membrane protein). K+ increased toxin binding at low concentrations but exerted opposite effects at high concentrations. Ca2+, guanidinium and Na+ exerted only inhibitory effects on binding. Scyllatoxin binding sites were overexpressed 2.5-fold after a 24-h cell pretreatment with 2 mM butyrate. This effect was suppressed by cycloheximide.

• Authors: D Gossen, J C Gesquière, M Tastenoy, P De Neef, M Waelbroeck, J Christophe
• Journal: FEBS letters (FEBS Lett) Vol. 285 Issue 2 Pg. 271-4 (Jul 22 1991) ISSN: 0014-5793 [Print] England
• PMID: 1855593 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Butyrates
• Guanidines
• Potassium Channels
• Receptors, Cholinergic
• Scorpion Venoms
• scyllatoxin receptor
• Butyric Acid
• leiurotoxin I
• Apamin
• Cycloheximide
• Sodium
• Guanidine
• Potassium
• Calcium

Topics

• Amino Acid Sequence
• Apamin (antagonists & inhibitors, metabolism)
• Butyrates (pharmacology)
• Butyric Acid
• Calcium (metabolism)
• Cell Membrane (metabolism)
• Cycloheximide (pharmacology)
• Gene Expression Regulation (drug effects)
• Guanidine
• Guanidines (metabolism)
• Humans
• Kinetics
• Molecular Sequence Data
• Neuroblastoma
• Potassium (metabolism)
• Potassium Channels
• Radioligand Assay
• Receptors, Cholinergic (drug effects, metabolism)
• Scorpion Venoms (metabolism)
• Sodium (metabolism)
• Tumor Cells, Cultured