Antioxidant properties of 2-O-beta-D-glucopyranosyl-L-ascorbic acid.

Abstract

The antioxidant activity of a provitamin C agent, 2-O-beta-D-glucopyranosyl-L-ascorbic acid (AA-2betaG), was compared to that of 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) and ascorbic acid (AA) using four in vitro methods, 1,1-diphenyl-picrylhydrazyl (DPPH) radical-scavenging assay, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(*+))-scavenging assay, oxygen radical absorbance capacity (ORAC) assay, and 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis inhibition assay. AA-2betaG slowly and continuously scavenged DPPH radicals and ABTS(*+) in roughly the same reaction profiles as AA-2G, whereas AA quenched these radicals immediately. In the ORAC assay and the hemolysis inhibition assay, AA-2betaG showed similar overall activities to AA-2G and to AA, although the reactivity of AA-2betaG against the peroxyl radical generated in both assays was lower than that of AA-2G and AA. These data indicate that AA-2betaG had roughly the same radical-scavenging properties as AA-2G, and a comprehensive in vitro antioxidant activity of AA-2betaG appeared to be comparable not only to that of AA-2G but also to that of AA.

• Authors: Jun Takebayashi, Yasuyuki Yagi, Rie Ishii, Shigeki Abe, Kazuhiko Yamada, Akihiro Tai
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 72 Issue 6 Pg. 1558-63 (Jun 2008) ISSN: 1347-6947 [Electronic] England
• PMID: 18540110 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2-O-(beta-D-glucopyranosyl)ascorbic acid
• Amidines
• Antioxidants
• Benzothiazoles
• Sulfonic Acids
• 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
• 2,2'-azobis(2-amidinopropane)
• Ascorbic Acid

Topics

• Amidines (pharmacology)
• Animals
• Antioxidants (pharmacology)
• Ascorbic Acid (analogs & derivatives, chemistry, pharmacology)
• Benzothiazoles (chemistry)
• Erythrocytes (drug effects)
• Hemolysis (drug effects)
• Hydrogen-Ion Concentration
• Molecular Structure
• Sheep
• Sulfonic Acids (chemistry)