Enhancement of immunostimulatory properties of exosomal vaccines by incorporation of fusion-competent G protein of vesicular stomatitis virus.

Exosomes have been proposed as candidates for therapeutic immunization. The present study demonstrates that incorporation of the G protein of vesicular stomatitis virus (VSV-G) into exosome-like vesicles (ELVs) enhances their uptake and induces the maturation of dendritic cells. Targeting of VSV-G and ovalbumin as a model antigen to the same ELVs increased the cross-presentation of ovalbumin via an endosomal acidification mechanism. Immunization of mice with VSV-G and ovalbumin containing ELVs led to an increased IgG2a antibody response, expansion of antigen-specific CD8 T cells, strong in vivo CTL responses, and protection from challenge with ovalbumin expressing tumor cells. Thus, incorporation of VSV-G and targeting of antigens to ELVs are attractive strategies to improve exosomal vaccines.
AuthorsVladimir V Temchura, Matthias Tenbusch, Godwin Nchinda, Ghulam Nabi, Bettina Tippler, Maryna Zelenyuk, Oliver Wildner, Klaus Uberla, Seraphin Kuate
JournalVaccine (Vaccine) Vol. 26 Issue 29-30 Pg. 3662-72 (Jul 4 2008) ISSN: 0264-410X [Print] Netherlands
PMID18538453 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies
  • G protein, vesicular stomatitis virus
  • Membrane Glycoproteins
  • Vaccines
  • Viral Envelope Proteins
  • Ovalbumin
  • Animals
  • Antibodies (blood)
  • Antigen Presentation
  • CD8-Positive T-Lymphocytes (immunology)
  • Cytotoxicity, Immunologic
  • Dendritic Cells (immunology)
  • Endosomes (metabolism)
  • Humans
  • Membrane Glycoproteins (immunology)
  • Mice
  • Mice, Inbred C57BL
  • Neoplasms (prevention & control)
  • Ovalbumin (immunology)
  • Protein Transport
  • Secretory Vesicles (immunology)
  • Vaccines (immunology)
  • Viral Envelope Proteins (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: