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Enhancement of immunostimulatory properties of exosomal vaccines by incorporation of fusion-competent G protein of vesicular stomatitis virus.

Abstract
Exosomes have been proposed as candidates for therapeutic immunization. The present study demonstrates that incorporation of the G protein of vesicular stomatitis virus (VSV-G) into exosome-like vesicles (ELVs) enhances their uptake and induces the maturation of dendritic cells. Targeting of VSV-G and ovalbumin as a model antigen to the same ELVs increased the cross-presentation of ovalbumin via an endosomal acidification mechanism. Immunization of mice with VSV-G and ovalbumin containing ELVs led to an increased IgG2a antibody response, expansion of antigen-specific CD8 T cells, strong in vivo CTL responses, and protection from challenge with ovalbumin expressing tumor cells. Thus, incorporation of VSV-G and targeting of antigens to ELVs are attractive strategies to improve exosomal vaccines.
AuthorsVladimir V Temchura, Matthias Tenbusch, Godwin Nchinda, Ghulam Nabi, Bettina Tippler, Maryna Zelenyuk, Oliver Wildner, Klaus Uberla, Seraphin Kuate
JournalVaccine (Vaccine) Vol. 26 Issue 29-30 Pg. 3662-72 (Jul 4 2008) ISSN: 0264-410X [Print] Netherlands
PMID18538453 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies
  • G protein, vesicular stomatitis virus
  • Membrane Glycoproteins
  • Vaccines
  • Viral Envelope Proteins
  • Ovalbumin
Topics
  • Animals
  • Antibodies (blood)
  • Antigen Presentation
  • CD8-Positive T-Lymphocytes (immunology)
  • Cytotoxicity, Immunologic
  • Dendritic Cells (immunology)
  • Endosomes (metabolism)
  • Humans
  • Membrane Glycoproteins (immunology)
  • Mice
  • Mice, Inbred C57BL
  • Neoplasms (prevention & control)
  • Ovalbumin (immunology)
  • Protein Transport
  • Secretory Vesicles (immunology)
  • Vaccines (immunology)
  • Viral Envelope Proteins (immunology)

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