T, not B, lymphocytes are required for immunodeficiency and paralysis induced by ts1, a mutant of Moloney murine leukemia virus-TB.

Abstract

BALB/c mice when injected as newborn with ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB, developed a fatal hindlimb paralysis and immunodeficiency. This disease induction was prevented, to a great extent, by transient depletion of the peripheral T lymphocytes during the early course of infection by using anti-Thy 1.2 antibody. FVB/N mice, which are highly susceptible to ts1, but express Thy 1.1 instead of Thy 1.2 on their T lymphocytes, did not show any difference in the disease profile when treated similarly with anti-Thy 1.2 antibody. Transient depletion of the peripheral B lymphocytes in BALB/c mice in the early course of ts1 infection had no effect on the disease induction. In the T cell depleted BALB/c mice, virus replication was reduced, survival of the mice was increased and viral specific antibodies were produced, whereas, in the B cell depleted mice the disease process went on in a fashion similar to untreated mice infected with ts1. Thus, this study demonstrates that the disease syndrome induced by ts1 in BALB/c mice is dependent upon the presence of T lymphocytes during the early course of infection, and that presence of B lymphocytes have little or no effect on the disease outcome.

• Authors: K Saha, P K Wong
• Journal: Virology (Virology) Vol. 183 Issue 2 Pg. 815-20 (Aug 1991) ISSN: 0042-6822 [Print] United States
• PMID: 1853579 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Topics

• Animals
• B-Lymphocytes (immunology)
• Fluorescent Antibody Technique
• Mice
• Mice, Inbred BALB C
• Moloney murine leukemia virus (genetics)
• Murine Acquired Immunodeficiency Syndrome (immunology, microbiology)
• Mutation
• T-Lymphocytes (immunology)
• Virus Replication (genetics)