HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

RNA interference silencing of DRAL affects processing of amyloid precursor protein.

Abstract
In a previous study, we reported that Alzheimer's disease-associated presenilin-2 interacts with a LIM-domain protein, namely, DRAL/FHL2/SLIM3. In this study, we investigated whether DRAL modifies the metabolism of the amyloid precursor protein (APP). We used small interfering RNA (siRNA) to knockdown DRAL in COS7 and HEK293 cells that stably overexpress APP695. We found that the knockdown was accompanied by a decrease in the amount of secreted alpha-secretase-cleaved APP and the membrane-bound C-terminal fragment C83 and an increase in the amount of secreted beta-amyloid peptide (Abeta) from the cells. We also found that in addition to a disintegrin and metalloprotease (ADAM)-17, DRAL binds to ADAM-10. Thus, DRAL may be involved in the processing of APP through the alpha-secretase pathway.
AuthorsHiroshi Tanahashi, Katsuji Yoshioka
JournalNeuroscience letters (Neurosci Lett) Vol. 439 Issue 3 Pg. 293-7 (Jul 18 2008) ISSN: 0304-3940 [Print] Ireland
PMID18534756 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amyloid beta-Protein Precursor
  • FHL2 protein, human
  • Homeodomain Proteins
  • LIM-Homeodomain Proteins
  • Membrane Proteins
  • Muscle Proteins
  • Phorbol Esters
  • Prostanoic Acids
  • RNA, Small Interfering
  • Transcription Factors
  • phorbol-12-myristate
  • Amyloid Precursor Protein Secretases
  • ADAM Proteins
  • ADAM10 Protein
  • ADAM10 protein, human
  • ADAM17 Protein
  • ADAM17 protein, human
  • rosaprostol
Topics
  • ADAM Proteins (metabolism)
  • ADAM10 Protein
  • ADAM17 Protein
  • Amyloid Precursor Protein Secretases (metabolism)
  • Amyloid beta-Protein Precursor (metabolism)
  • Animals
  • Cell Line, Transformed
  • Chlorocebus aethiops
  • Cricetinae
  • Homeodomain Proteins (metabolism)
  • Humans
  • LIM-Homeodomain Proteins
  • Membrane Proteins (metabolism)
  • Muscle Proteins (metabolism)
  • Phorbol Esters (pharmacology)
  • Prostanoic Acids (metabolism)
  • RNA, Small Interfering (metabolism)
  • Transcription Factors (metabolism)
  • Transfection

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: