[Expression and distribution of monocyte chemotactic protein-3 and its significance in laryngeal carcinoma].

Abstract

OBJECTIVE:

To investigate monocyte chemotactic protein-3 (MCP-3) mRNA and protein expression and distribution in laryngeal squamous cell carcinoma (LSCC) tissue, so as to study its function in the tumor development.

METHOD:

Immunohistochemical staining and RT-PCR, and western blot were performed to detect the LSCC tissue and the normal tissue adjacent tumor.

RESULT:

The expression of MCP-3 mRNA and protein were found in the LSCC tissue and the normal tissue adjacent tumor. The expression of MCP-3 mRNA and protein in LSCC tissue is much higher than that in normal tissue adjacent tumor (P<0.05). There is a significant difference between I-II stage III-IV stage (P<0.05). Immunohistochemical staining: positive staining of MCP-3 was yellow and mainly detected in the tumor cell cytoplasm and also in some normal glandular epitheliums.

CONCLUSION:

MCP-3 might be involved in the mechanism of the tumor development, its function of chemotaxis and activation of the tumor-associated macrophages would be highlighted, so that the inflammatory microenvironment might play an important role in the development of tumor.

• Authors: Lin Li, Dongdong Zhu, Zhen Dong
• Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery (Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 22 Issue 6 Pg. 244-7 (Mar 2008) ISSN: 2096-7993 [Print] China
• PMID: 18533557 (Publication Type: English Abstract, Journal Article)

Chemical References

• CCL7 protein, human
• Chemokine CCL7
• RNA, Messenger

Topics

• Adult
• Aged
• Carcinoma, Squamous Cell (metabolism, pathology)
• Chemokine CCL7 (metabolism)
• Female
• Humans
• Laryngeal Neoplasms (metabolism, pathology)
• Male
• Middle Aged
• RNA, Messenger (genetics)