Effects of N-hexacosanol on nitric oxide synthase system in diabetic rat nephropathy.

Abstract

We attempted to clarify the effects of cyclohexenonic long-chain fatty alcohol (N-hexacosanol) on nitric oxide synthase (NOS) in streptozotocin-induced diabetic nephropathy. After induction of experimental diabetes with streptozotocin, rats were maintained for 8 weeks with or without treatment by N-hexacosanol (8 mg/kg i.p. every day). Urinary albumin excretion, blood chemistry, immunoblot analysis, and real-time polymerase chain reactions (real-time PCR) of endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), and neuronal NOS (nNOS) were investigated. Although N-hexacosanol had no effects on serum glucose or insulin level, it normalized serum creatinine and urinary albumin excretion. N-hexacosanol was found to improve the diabetes-induced alterations in the eNOS, iNOS, and nNOS protein and their mRNA levels. Histologically, N-hexacosanol inhibited the progression to glomerular sclerosis. Our data suggest that N-hexacosanol improves diabetes-induced NOS alterations in the kidney, resulting in the amelioration of diabetic nephropathy.

• Authors: Shinichi Okada, Motoaki Saito, Emi Kazuyama, Takuya Hanada, Yasuo Kawaba, Atsushi Hayashi, Keisuke Satoh, Susumu Kanzaki
• Journal: Molecular and cellular biochemistry (Mol Cell Biochem) Vol. 315 Issue 1-2 Pg. 169-77 (Aug 2008) ISSN: 0300-8177 [Print] Netherlands
• PMID: 18528636 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fatty Alcohols
• Nitrates
• Nitrites
• RNA, Messenger
• Nitric Oxide Synthase
• Nitric Oxide Synthase Type I
• Nitric Oxide Synthase Type II
• Nitric Oxide Synthase Type III
• 1-hexacosanol

Topics

• Animals
• Diabetic Nephropathies (enzymology, pathology)
• Fatty Alcohols (pharmacology)
• Gene Expression Regulation, Enzymologic (drug effects)
• Immunoblotting
• Kidney (drug effects, enzymology, pathology)
• Male
• Nitrates (metabolism)
• Nitric Oxide Synthase (genetics, metabolism)
• Nitric Oxide Synthase Type I (genetics, metabolism)
• Nitric Oxide Synthase Type II (genetics, metabolism)
• Nitric Oxide Synthase Type III (genetics, metabolism)
• Nitrites (metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley