Preventive effects of a Kampo medicine, Shosaikoto, on inflammatory responses in LPS-treated human gingival fibroblasts.

Abstract

In the present study, we investigated the anti-inflammatory effects of a Kampo medicine Shosaikoto (TJ-9) using in vitro periodontal disease model, in which human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis (PgLPS) produce IL-6, IL-8 and prostaglandin E2 (PGE2). Treatment with PgLPS (10 ng/ml), TJ-9 (up to 1 mg/ml) and their combinations for 24 h did not affect the viability of HGFs. Moreover, TJ-9 did not alter LPS-induced IL-6 and IL-8 productions. However, TJ-9 significantly suppressed LPS-induced PGE2 production in a dose-dependent manner but TJ-9 alone did not affect basal PGE2 level. Western blotting demonstrated that TJ-9 decreased cyclooxygenase-2 (COX-2) expression in a dose-dependent manner but not phospholipase A2. Moreover, TJ-9 selectively and dose-dependently inhibited COX-2 activity. These results suggest that TJ-9 decreased PGE2 production by inhibition of both COX-2 expression and activity and that TJ-9 may be useful to improve gingival inflammation in periodontal disease.

• Authors: Toshiaki Ara, Yoshihiro Maeda, Yoshiaki Fujinami, Yasuhiro Imamura, Toshimi Hattori, Pao-Li Wang
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 31 Issue 6 Pg. 1141-4 (Jun 2008) ISSN: 0918-6158 [Print] Japan
• PMID: 18520044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Drugs, Chinese Herbal
• Indicators and Reagents
• Interleukin-6
• Interleukin-8
• Lipopolysaccharides
• Tetrazolium Salts
• Thiazoles
• shosaikoto-go-keishika-shakuyakuto
• Cyclooxygenase 1
• Cyclooxygenase 2
• thiazolyl blue
• Dinoprostone

Topics

• Anti-Inflammatory Agents, Non-Steroidal
• Blotting, Western
• Cell Survival (drug effects)
• Cyclooxygenase 1 (metabolism)
• Cyclooxygenase 2 (metabolism)
• Dinoprostone (biosynthesis)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology)
• Enzyme-Linked Immunosorbent Assay
• Fibroblasts (drug effects)
• Gingiva (cytology, drug effects)
• Gingivitis (drug therapy, pathology)
• Humans
• Indicators and Reagents
• Interleukin-6 (biosynthesis)
• Interleukin-8 (biosynthesis)
• Lipopolysaccharides (antagonists & inhibitors, toxicity)
• Macrophages (drug effects, metabolism)
• Medicine, Kampo
• Monocytes (drug effects, metabolism)
• Porphyromonas gingivalis (chemistry)
• Tetrazolium Salts
• Thiazoles