Abstract | BACKGROUND:
Benzoylmesaconine (BMA) is the main Aconitum alkaloid in Radix Aconiti Lateralis Preparata ( Fuzi, aconite roots) with potent pharmacological activities, such as analgesia and anti- inflammation. The present study developed a simple and reliable method using BMA as a marker compound for the quality control of processed aconite roots and their products. METHODS: After extraction, a high-performance liquid chromatography (HPLC) determination of BMA was conducted on a RP-C18 column by gradient elution with acetonitrile and aqueous phase, containing 0.1% phosphoric acid adjusted with triethylamine to pH 3.0. RESULTS: A distinct peak profile was obtained and separation of BMA was achieved. Method validation showed that the relative standard deviations (RSDs) of the precision of BMA in all intra-day and inter-day assays were less than 1.36%, and that the average recovery rate was 96.95%. Quantitative analysis of BMA showed that the content of BMA varied significantly in processed aconite roots and their products. CONCLUSION: This HPLC method using BMA as a marker compound is applicable to the quality control of processed aconite roots and their products.
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Authors | Ying Xie, Hua Zhou, Yuen Fan Wong, Zhongqiu Liu, Hongxi Xu, Zhihong Jiang, Liang Liu |
Journal | Chinese medicine
(Chin Med)
Vol. 3
Pg. 6
(May 30 2008)
ISSN: 1749-8546 [Electronic] England |
PMID | 18513409
(Publication Type: Journal Article)
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