T-cell responses in osteosarcoma patients vaccinated with an anti-idiotypic antibody, 105AD7, mimicking CD55.

Abstract

We assessed T-cell responses in young osteosarcoma patients vaccinated with 105AD7, 1-6 months after having received chemotherapy. 105AD7 is a human anti-idiotypic antibody mimicking CD55, a glycoprotein that protects from attack by complement and which is overexpressed on osteosarcoma cells. Seven out of 21 investigated patients made a IFN-gamma T-cell response against the vaccine, 105AD7 as assessed by ELISPOT. Cytokine secretion was analysed using Luminex assays and revealed TNF-alpha and GM-CSF responses not only to the vaccine but also towards the native antigen, CD55, in 5 / 14 (36%) of investigated patients. Importantly, the Luminex assay was found to be more sensitive than the more established T-cell assays (ELISPOT and proliferation assay), since responses towards the native antigen were recorded in this assay. Clinical responses and induction of immune responses to both the anti-idiotype and the native CD55 antigen support the use of CD55 as a target in cancer treatment.

• Authors: G J Ullenhag, I Spendlove, N F S Watson, C Kallmeyer, K Pritchard-Jones, L G Durrant
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 128 Issue 2 Pg. 148-54 (Aug 2008) ISSN: 1521-7035 [Electronic] United States
• PMID: 18508409 (Publication Type: Clinical Trial, Clinical Trial, Phase I, Clinical Trial, Phase II, Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't)

Chemical References

• 105AD7 antibody
• Antibodies, Anti-Idiotypic
• Antibodies, Monoclonal
• CD55 Antigens
• Cancer Vaccines
• Tumor Necrosis Factor-alpha
• Interferon-gamma
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Antibodies, Anti-Idiotypic (administration & dosage, immunology)
• Antibodies, Monoclonal (administration & dosage, immunology)
• Bone Neoplasms (immunology)
• CD55 Antigens (immunology)
• Cancer Vaccines (administration & dosage, immunology)
• Cell Proliferation
• Granulocyte-Macrophage Colony-Stimulating Factor (biosynthesis, immunology)
• Humans
• Immunization, Passive
• Immunoenzyme Techniques
• Injections, Intramuscular
• Injections, Subcutaneous
• Interferon-gamma (biosynthesis, immunology)
• Osteosarcoma (immunology)
• T-Cell Antigen Receptor Specificity
• T-Lymphocytes (immunology, metabolism)
• Treatment Outcome
• Tumor Necrosis Factor-alpha (biosynthesis, immunology)
• United Kingdom