Comparison of apolipoprotein D determination methods in breast cancer.

Abstract

BACKGROUND:

Apolipoprotein D (ApoD) is a promising prognostic and predictive factor in breast cancer, but the analysis methods and results vary.

PATIENTS AND METHODS:

Determination of ApoD content by immunoelectrophoresis in tumour cytosol (EPC), immunohistochemistry (IHC) in whole sections (WS) and tissue micro arrays (TMA) were compared in 283 breast carcinomas.

RESULTS:

With EPC, 45% and with IHC, 71% of the tumours were ApoD-positive. Correlation between the degrees of ApoD positivity by ECP and IHC was poor (R2=0.04), caused by higher sensitivity of the IHC (resulting in many ECP negative carcinomas being IHC positive) and ApoD positivity of normal tissues and cysts (resulting in ApoD positivity by ECP in up to 33% of the IHC negative cases). Discrepancies between WS and TMA were considerable due to tumour heterogeneity.

CONCLUSION:

In breast cancer, IHC ApoD determination is superior to EPC analysis, but intratumor heterogeneity must be carefully considered when using TMA technology.

• Authors: Håvard Søiland, Ivar Skaland, Emiel A M Janssen, Einar Gudlaugsson, Hartwig Körner, Jan Erik Varhaug, Jon Arne Søreide, Jan P A Baak
• Journal: Anticancer research (Anticancer Res) 2008 Mar-Apr Vol. 28 Issue 2B Pg. 1151-60 ISSN: 0250-7005 [Print] Greece
• PMID: 18505051 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• APOD protein, human
• Apolipoproteins D
• Biomarkers, Tumor
• Glycoproteins
• Membrane Transport Proteins

Topics

• Aged
• Apolipoproteins D (analysis, metabolism)
• Biomarkers, Tumor (analysis, metabolism)
• Breast Neoplasms (chemistry, metabolism, pathology)
• Cytosol (chemistry, metabolism)
• Female
• Glycoproteins (analysis, metabolism)
• Humans
• Immunoelectrophoresis (methods)
• Immunohistochemistry (methods)
• Membrane Transport Proteins (analysis, metabolism)
• Microarray Analysis (methods)
• Middle Aged
• Staining and Labeling (methods)