Abstract | OBJECTIVE: The selective loss of dopaminergic neurons in Parkinson's disease is suspected to correlate with the increase of cellular iron, which may be involved in the pathogenesis of PD by promotion of oxidative stress. This research investigated dopamine-induced oxidative stress toxicity contributed by iron and the production of dopamine-derived neurotoxins in dopaminergic SH-SY5Y cells. METHODS: RESULTS: (1) Optimal dopamine (150 micromol/L) and Fe2+ (40 or 80 micromol/L) significantly increased the concentrations of hydroxy radicals and melondialdehyde in SH-SY5Y cells. (2) Induction with dopamine alone or dopamine and Fe2+ ( dopamine/Fe2+) caused cell apoptosis. (3) Compared with untreated cells, the catechol isoquinolines, salsolinol and N-methyl- salsolinol in dopamine/Fe2+-induced cells were detected in increasing amounts. CONCLUSION:
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Authors | Ran Wang, Hong Qing, Xiao-Qian Liu, Xiao-Lin Zheng, Yu-Lin Deng |
Journal | Neuroscience bulletin
(Neurosci Bull)
Vol. 24
Issue 3
Pg. 125-32
(Jun 2008)
ISSN: 1673-7067 [Print] Singapore |
PMID | 18500384
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Catechols
- Isoquinolines
- Neurotoxins
- Salsoline Alkaloids
- Hydroxyl Radical
- Malondialdehyde
- salsolinol
- salsoline
- Iron
- Dopamine
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Topics |
- Apoptosis
(drug effects, physiology)
- Catechols
(metabolism)
- Cell Line, Tumor
- Cell Survival
(drug effects, physiology)
- Dopamine
(toxicity)
- Dose-Response Relationship, Drug
- Humans
- Hydroxyl Radical
(metabolism)
- Iron
(metabolism)
- Iron Metabolism Disorders
(complications, metabolism, physiopathology)
- Isoquinolines
(metabolism)
- Malondialdehyde
(metabolism)
- Models, Biological
- Nerve Degeneration
(chemically induced, metabolism, physiopathology)
- Neurons
(drug effects, metabolism)
- Neurotoxins
(toxicity)
- Oxidative Stress
(drug effects)
- Parkinson Disease
(etiology, metabolism, physiopathology)
- Salsoline Alkaloids
(metabolism)
- Up-Regulation
(drug effects, physiology)
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