Abstract |
A method has been developed for the measurement of plasma concentrations of Beta-cell tropin (BCT), which is a potent insulinotropic and lipogenic peptide secreted by the pituitary. The method was employed to compare plasma Beta-cell tropin concentrations between lean and genetically obese (ob/ob) mice and between lean and genetically obese (fa/fa) Zucker rats. The plasma concentration in lean mice was 0.17 +/- 0.02 (5)nmole/l (mean +/- SEM, n = 5), while that in obese (ob/ob) mice was significantly higher, being 2.88 +/- 1.13 (5)nmole/l. The plasma BCT concentration in Zucker rats was 0.14 +/- 0.02 (15)nmole/l, while that in obese Zucker (fa/fa) rats was significantly higher, being 1.69 +/- 0.72 (16)nmole/l. These results explain previously observed differences in the Beta-cell tropin-like biological activity in plasma from lean and obese animals, and support the hypothesis that the peptide has a role in the development of hyperinsulinaemia and obesity.
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Authors | J L Morton, M Davenport, S J Dunmore |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 174
Issue 2
Pg. 767-71
(Jan 31 1991)
ISSN: 0006-291X [Print] United States |
PMID | 1847053
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Peptide Fragments
- beta-cell tropin
- Adrenocorticotropic Hormone
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Topics |
- Acetylation
- Adrenocorticotropic Hormone
(blood, isolation & purification)
- Animals
- Chromatography, High Pressure Liquid
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Obese
(blood)
- Peptide Fragments
(blood, isolation & purification)
- Radioimmunoassay
- Rats
- Rats, Zucker
(blood)
- Reference Values
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