[Effects of genistein on the expressions of cell apoptosis-related proteins in salivary adenoid cystic carcinoma cell line SACC-83].

Abstract

PURPOSE:

To investigate the molecular mechanism of cell apoptosis induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83.

METHODS:

SACC-83 cells cultured in vitro were treated with genistein, the expressions of bax, bcl-2 and survivin proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.5 software package.

RESULTS:

With the increase of concentration of genistein and the elongation of time, the expression of bax protein was significantly increased, but the expression of bcl-2 and survivin proteins was significantly decreased. Treated with 220 micromol/L genistein for 3 days, the expression level of bax protein was 3.43 folds of the control group (P<0.01),but the expression level of bcl-2 and survivin proteins was respectively 85%(P<0.05) and 35%(P<0.01) of the control group.

CONCLUSION:

The cell apoptosis induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the upregulation of bax protein expression,and the downregulations of bcl-2 and survivin proteins expression.

• Authors: Jie Ma, Jie Wang, Ming Zhong, Zhao-Yuan Wang
• Journal: Shanghai kou qiang yi xue = Shanghai journal of stomatology (Shanghai Kou Qiang Yi Xue) Vol. 17 Issue 2 Pg. 196-9 (Apr 2008) ISSN: 1006-7248 [Print] China
• PMID: 18470428 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteins
• Genistein

Topics

• Apoptosis (drug effects)
• Carcinoma, Adenoid Cystic (metabolism)
• Cell Line
• Cell Line, Tumor
• Gene Expression Regulation (drug effects)
• Genistein (pharmacology)
• Humans
• Proteins (metabolism)
• Salivary Gland Neoplasms (metabolism)