Abstract |
This study reports on the predictive relationship between serological, immunological and pathological responses following experimental inoculation with incremental doses of Fasciola gigantica in sheep. Fifty, 6-month-old, naive Merino wethers were allocated to one of 5 experimental groups, four of which received 50, 125, 225 and 400 metacercariae, respectively, whilst a 5th group acted as non-inoculated control. Strong individual correlations were observed between liver score, GLDH ( glutamate dehydrogenase), GGT (gamma glutamyl transferase), CatL5 ( cathepsin L5) antibody titre ( IgG1, IgA), eosinophilia, and the total worm count or worm biomass. A combination of multiple indicator traits performed significantly better than any single indicator trait alone. The best predictive index accounted for up to 88% of observed worm burden (Wb) if information on inoculation dose was available. Without knowledge of inoculation dose, such as under field conditions, up to 67% of variation in worm burden could be predicted. In contrast, the best single predictor variable (liver damage score) accounted for up to 50% of worm burden, and in the absence of post-slaughter information, serum levels of anti- cathepsin IgA antibody titres accounted for 35% of predicted variation in worm burden. The utility of a predictive index under both field and experimental inoculation conditions is discussed.
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Authors | H W Raadsma, N M Kingsford, Suharyanta, T W Spithill, D Piedrafita |
Journal | Veterinary parasitology
(Vet Parasitol)
Vol. 154
Issue 3-4
Pg. 250-61
(Jul 04 2008)
ISSN: 0304-4017 [Print] Netherlands |
PMID | 18468797
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glutamine
- Oxidoreductases
- Acyltransferases
- glutamine phenylacetyltransferase
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Topics |
- Acyltransferases
(blood)
- Animals
- Fasciola
(physiology)
- Fascioliasis
(parasitology, veterinary)
- Glutamine
(metabolism)
- Host-Parasite Interactions
- Male
- Models, Biological
- Oxidoreductases
(blood, metabolism)
- Predictive Value of Tests
- Serologic Tests
- Sheep
- Sheep Diseases
(immunology, parasitology)
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