The Escherichia coli mraY gene encoding UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase.

Abstract

Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the formation of undecaprenyl-pyrophosphoryl-N-acetylmuramoyl-pentapeptide from UDP-N-acetylmuramoyl-pentapeptide and undecaprenyl-phosphate, the first step in the lipid cycle reactions in biosynthesis of bacterial cell wall peptidoglycans. The enhanced enzyme activity was sensitive to tunicamycin, and the amino tunicamycin-sensitive N-acetylglucosamine-1-phosphate transferase of Saccharomyces cerevisiae. Very probably mraY is the structural gene for the above enzyme.

• Authors: M Ikeda, M Wachi, H K Jung, F Ishino, M Matsuhashi
• Journal: Journal of bacteriology (J Bacteriol) Vol. 173 Issue 3 Pg. 1021-6 (Feb 1991) ISSN: 0021-9193 [Print] United States
• PMID: 1846850 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Uridine Diphosphate N-Acetylmuramic Acid
• Tunicamycin
• UDP-N-acetylmuramic acid pentapeptide
• Uridine Monophosphate
• Phosphotransferases
• Transferases (Other Substituted Phosphate Groups)
• phospho-N-acetylmuramoyl pentapeptide transferase
• UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase

Topics

• Amino Acid Sequence
• Escherichia coli (enzymology, genetics)
• Genes, Bacterial
• Kinetics
• Molecular Sequence Data
• Phosphotransferases (chemistry, genetics, metabolism)
• Plasmids
• Restriction Mapping
• Saccharomyces cerevisiae (enzymology, genetics)
• Sequence Homology, Nucleic Acid
• Transferases (Other Substituted Phosphate Groups)
• Tunicamycin (pharmacology)
• Uridine Diphosphate N-Acetylmuramic Acid (analogs & derivatives, metabolism)
• Uridine Monophosphate (metabolism)