C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.

Abstract

In Staphylococcus aureus, the target of RNAIII activating protein (TRAP) is a membrane-associated protein whose C-terminus can be used as a vaccine to provide protection against staphylococcal infection. Here, we show for the first time by surface plasmon resonance and enzyme-linked immunosorbent assay that TRAP can specifically bind lysozyme and lysostaphin through its C-terminus (amino acids 155-167) and enhance lysozomal activities in vitro. It was also found that the traP mutant strain is more resistant to lysostaphin than wild-type. Our previous data showed that the C-terminus of TRAP might be extracellular. So our results suggested that the C-terminus of TRAP could act as the specific targeting protein of the lysozyme/lysostaphin on the S. aureus cell wall and the biological significance of the interaction might be to facilitate lysozyme/lysostaphin-mediated cell lysis.

• Authors: Guang Yang, Yaping Gao, Jiannan Feng, Yong Huang, Shaohua Li, Yu Liu, Chuan Liu, Ming Fan, Beifen Shen, Ningsheng Shao
• Journal: Acta biochimica et biophysica Sinica (Acta Biochim Biophys Sin (Shanghai)) Vol. 40 Issue 5 Pg. 452-8 (May 2008) ISSN: 1745-7270 [Electronic] China
• PMID: 18465031 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• MtrB protein, Bacteria
• RNA-Binding Proteins
• Transcription Factors
• Muramidase
• Lysostaphin

Topics

• Bacterial Proteins (chemistry)
• Binding Sites
• Enzyme Activation
• Lysostaphin (chemistry)
• Muramidase (chemistry)
• Protein Binding
• Protein Structure, Tertiary
• RNA-Binding Proteins (chemistry)
• Staphylococcus aureus (metabolism)
• Transcription Factors (chemistry)