IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine.

Abstract

IL-7 and IL-15 are key cytokines involved in the generation and maintenance of memory CD8+ T-cells. We evaluated these cytokines as molecular adjuvants for topical HIV-1 DermaVir vaccine. We found that mice receiving DermaVir formulated with HIV-1 Gag plasmid in the presence of IL-7- or IL-15-encoding plasmid significantly enhanced Gag-specific central memory T-cells, as measured by a peptide-based cultured IFN-gamma ELISPOT. Additionally, IL-15 significantly improved DermaVir-induced Gag-specific effector memory CD8+ T-cell responses, measured by standard IFN-gamma ELISPOT. In a DermaVir prime/vaccinia vector boost regimen, the inclusion of IL-15 together with DermaVir significantly improved Gag-specific effector memory T-cell responses. Our study demonstrates that IL-15 is more potent than IL-7 in enhancing HIV-1-specific central memory T-cells induced by topical DermaVir. IL-15 adjuvanted DermaVir might be an alternative prime in a prophylactic vaccine regimen.

• Authors: Sandra A Calarota, Anlan Dai, Jeffrey N Trocio, David B Weiner, Franco Lori, Julianna Lisziewicz
• Journal: Vaccine (Vaccine) Vol. 26 Issue 40 Pg. 5188-95 (Sep 19 2008) ISSN: 0264-410X [Print] Netherlands
• PMID: 18462844 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• AIDS Vaccines
• Adjuvants, Immunologic
• DermaVir
• Gene Products, gag
• Interleukin-15
• Interleukin-7

Topics

• AIDS Vaccines (administration & dosage, immunology)
• Adjuvants, Immunologic
• Administration, Topical
• Animals
• CD8-Positive T-Lymphocytes (immunology)
• Female
• Gene Products, gag (immunology)
• HIV Infections (immunology, prevention & control, virology)
• HIV-1 (immunology)
• Immunologic Memory
• Interleukin-15 (administration & dosage, immunology)
• Interleukin-7 (administration & dosage, immunology)
• Mice
• Mice, Inbred BALB C
• T-Lymphocytes (immunology)
• Vaccination