Abstract | BACKGROUND: The TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) mouse model has frequently been used in preclinical studies with chemotherapeutic/chemopreventive rationales. Here the hypothesis was tested using (1)H-NMR-based metabolic profiling that the TRAMP tumor metabolic phenotype resembles that reported for human prostate cancer. METHODS: Aqueous extracts or intact tissues of normal prostate from 8- ("young") or 28-("old") week-old C57BL/6J wild-type mice or of prostate tumor from age-matched TRAMP mice were analyzed by (1)H-NMR. Results were compared with immunohistochemical findings. Expression of choline kinase was studied at the protein and mRNA levels. RESULTS: CONCLUSION:
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Authors | Friederike Teichert, Richard D Verschoyle, Peter Greaves, Richard E Edwards, Orla Teahan, Donald J L Jones, Ian D Wilson, Peter B Farmer, William P Steward, Timothy W Gant, Andreas J Gescher, Hector C Keun |
Journal | The Prostate
(Prostate)
Vol. 68
Issue 10
Pg. 1035-47
(Jul 01 2008)
ISSN: 0270-4137 [Print] United States |
PMID | 18459103
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | (c) 2008 Wiley-Liss, Inc. |
Chemical References |
- Biomarkers, Tumor
- Phosphatidylcholines
- Phospholipids
- Protons
- Phosphorylcholine
- Glycerylphosphorylcholine
- Choline Kinase
- Choline
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Topics |
- Adenocarcinoma
(genetics, metabolism, pathology)
- Animals
- Biomarkers, Tumor
- Choline
(metabolism)
- Choline Kinase
(genetics, metabolism)
- Disease Models, Animal
- Gene Expression Regulation, Neoplastic
- Glycerylphosphorylcholine
(metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Nuclear Magnetic Resonance, Biomolecular
(methods)
- Oligonucleotide Array Sequence Analysis
- Phosphatidylcholines
(metabolism)
- Phospholipids
(metabolism)
- Phosphorylcholine
(metabolism)
- Prostate
(metabolism, pathology)
- Prostatic Neoplasms
(genetics, metabolism, pathology)
- Protons
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