Acacetin, a natural flavone, selectively inhibits human atrial repolarization potassium currents and prevents atrial fibrillation in dogs.

Abstract

BACKGROUND:

The development of atrium-selective antiarrhythmic agents is a current strategy for inhibiting atrial fibrillation (AF). The present study investigated whether the natural flavone acacetin from the traditional Chinese medicine Xuelianhua would be an atrium-selective anti-AF agent.

METHODS AND RESULTS:

The effects of acacetin on human atrial ultrarapid delayed rectifier K(+) current (I(Kur)) and other cardiac ionic currents were studied with a whole-cell patch technique. Acacetin suppressed I(Kur) and the transient outward K(+) current (IC(50) 3.2 and 9.2 mumol/L, respectively) and prolonged action potential duration in human atrial myocytes. The compound blocked the acetylcholine-activated K(+) current; however, it had no effect on the Na(+) current, L-type Ca(2+) current, or inward-rectifier K(+) current in guinea pig cardiac myocytes. Although acacetin caused a weak reduction in the hERG and hKCNQ1/hKCNE1 channels stably expressed in HEK 293 cells, it did not prolong the corrected QT interval in rabbit hearts. In anesthetized dogs, acacetin (5 mg/kg) prolonged the atrial effective refractory period in both the right and left atria 1 to 4 hours after intraduodenal administration without prolongation of the corrected QT interval, whereas sotalol at 5 mg/kg prolonged both the atrial effective refractory period and the corrected QT interval. Acacetin prevented AF induction at doses of 2.5 mg/kg (50%), 5 mg/kg (85.7%), and 10 mg/kg (85.7%). Sotalol 5 mg/kg also prevented AF induction (60%).

CONCLUSIONS:

The present study demonstrates that the natural compound acacetin is an atrium-selective agent that prolongs the atrial effective refractory period without prolonging the corrected QT interval and effectively prevents AF in anesthetized dogs after intraduodenal administration. These results indicate that oral acacetin is a promising atrium-selective agent for the treatment of AF.

• Authors: Gui-Rong Li, Hong-Bing Wang, Guo-Wei Qin, Man-Wen Jin, Qiang Tang, Hai-Ying Sun, Xin-Ling Du, Xiu-Ling Deng, Xiao-Hua Zhang, Jing-Bo Chen, Lei Chen, Xiao-Hui Xu, Lik-Cheung Cheng, Shui-Wah Chiu, Hung-Fat Tse, Paul M Vanhoutte, Chu-Pak Lau
• Journal: Circulation (Circulation) Vol. 117 Issue 19 Pg. 2449-57 (May 13 2008) ISSN: 1524-4539 [Electronic] United States
• PMID: 18458165 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Arrhythmia Agents
• Flavones
• acacetin
• Potassium

Topics

• Action Potentials (drug effects)
• Animals
• Anti-Arrhythmia Agents (pharmacology)
• Atrial Fibrillation (drug therapy, prevention & control)
• Atrial Function (drug effects)
• Cells, Cultured
• Flavones (pharmacology, therapeutic use)
• Guinea Pigs
• Humans
• Medicine, Chinese Traditional
• Myocytes, Cardiac
• Patch-Clamp Techniques
• Potassium (metabolism)