Abstract | OBJECTIVES: METHODS: We have examined the effect of DL- propargylglycine, a cystathionine gamma-lyase inhibitor, on the synthesis of CC chemokine monocyte chemotactic protein 1, Regulated upon Activation, Normal T-cell Expressed, and Secreted, and macrophage inflammatory protein-1alpha (MIP-1alpha), and CXC chemokine MIP-2 in an in vitro and in vivo model of cerulein-induced acute pancreatitis and associated lung injury. In addition, the pancreatic acinar cells were treated with H2S donor drug, sodium hydrosulfide. The expression of these chemokines in the pancreatic acini, pancreas, and lungs was determined by quantitative real-time reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. RESULTS: CONCLUSIONS: These results suggest that the proinflammatory effect of H2S may be mediated by chemokines.
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Authors | Ramasamy Tamizhselvi, Philip K Moore, Madhav Bhatia |
Journal | Pancreas
(Pancreas)
Vol. 36
Issue 4
Pg. e24-31
(May 2008)
ISSN: 1536-4828 [Electronic] United States |
PMID | 18437075
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alkynes
- Chemokine CCL5
- Chemokines
- DNA Primers
- propargylglycine
- Ceruletide
- Cystathionine gamma-Lyase
- Glycine
- Hydrogen Sulfide
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Topics |
- Acute Disease
- Alkynes
(therapeutic use)
- Animals
- Ceruletide
(pharmacology, toxicity)
- Chemokine CCL5
(genetics)
- Chemokines
(antagonists & inhibitors)
- Cystathionine gamma-Lyase
(antagonists & inhibitors)
- DNA Primers
- Glycine
(analogs & derivatives, therapeutic use)
- Hydrogen Sulfide
(adverse effects)
- Male
- Mice
- Pancreatitis
(chemically induced, prevention & control)
- Respiratory Distress Syndrome
(chemically induced, prevention & control)
- Reverse Transcriptase Polymerase Chain Reaction
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