Targeting efficiency and biodistribution of biotinylated-EGF-conjugated gelatin nanoparticles administered via aerosol delivery in nude mice with lung cancer. | CureHunter

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Targeting efficiency and biodistribution of biotinylated-EGF-conjugated gelatin nanoparticles administered via aerosol delivery in nude mice with lung cancer.

Abstract	Lung cancer is the most malignant cancer today; in order to develop an effective drug delivery system for lung cancer therapy, gelatin nanoparticles (GPs) were modified with NeutrAvidin(FITC)-biotinylated epidermal growth factor (EGF) to form EGF receptor (EGFR)-seeking nanoparticles (GP-Av-bEGF). Aerosol droplets of the GP-Av-bEGF were generated by using a nebulizer and were delivered to mice model of lung cancer via aerosol delivery. Analysis of the aerosol size revealed that 99% of the nanoparticles after nebulization had a mass median aerodynamic diameter (MMAD) within the suitable range (0.5-5 microm) for lower airway deposition. The safety of inhaled nanoparticles was examined by lung edema and myeloperoxidase (MPO) activity assay. There's no finding suggestive of acute lung inflammation following inhalation. The fluorescence images obtained from live mice showed that the GP-Av-bEGF could target the cancerous lungs in a more specific manner. Fluorescence analysis of the organs revealed that the GP-Av-bEGF was mainly distributed in cancerous lungs. In contrast, nanoparticle accumulation was lower in normal lungs. The histological results indicated that the fluorescent GP-Av-bEGF was colocalized with the anti-EGFR-immunostain due to EGFR binding. The results of this study revealed that GP-Av-bEGF could target to the EGFR-overexpression cancer cells in vivo and may prove to be beneficial drug carriers when administered by simple aerosol delivery for the treatment of lung cancer.
Authors	Ching-Li Tseng, Steven Yueh-Hsiu Wu, Wen-Hsi Wang, Cheng-Liang Peng, Feng-Huei Lin, Chien-Cheng Lin, Tai-Horng Young, Ming-Jium Shieh
Journal	Biomaterials (Biomaterials) Vol. 29 Issue 20 Pg. 3014-22 (Jul 2008) ISSN: 0142-9612 [Print] Netherlands
PMID	18436301 (Publication Type: Journal Article)
Chemical References	Aerosols Epidermal Growth Factor Biotin Gelatin
Topics	Aerosols Animals Biotin (metabolism) Epidermal Growth Factor (administration & dosage, pharmacokinetics) Gelatin (chemistry) Lung Neoplasms (metabolism) Male Mice Mice, Nude Microscopy, Electron, Scanning Nanoparticles Tissue Distribution

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