Pattern recognition molecule mindin promotes intranasal clearance of influenza viruses.

Abstract

The innate immune response is essential for host defense against microbial pathogen infections and is mediated by pattern recognition molecules recognizing pathogen-associated molecular patterns. Our previous work has demonstrated that the extracellular matrix protein mindin functions as a pattern recognition molecule for bacterial pathogens. In this study, we examined the role of mindin in influenza virus infection. We found that intranasal infection of mindin-deficient mice by influenza virus resulted in dramatically increased virus titers in the lung and intranasal cavity of mutant mice. In contrast, lungs from intratracheally infected mindin-deficient mice contained similar influenza virus titers. We showed that mindin interacted with influenza virus particles directly and that mindin-deficient macrophages exhibited impaired activation after influenza virus infection in vitro. Furthermore, intranasal administration of recombinant mindin significantly enhanced the clearance of influenza virus in wild-type mice. Together, these results demonstrate that mindin plays an essential role in the host innate immune response to influenza virus infection and suggest that mindin may be used as an immune-enhancing agent in influenza infection.

• Authors: Wei Jia, Hong Li, You-Wen He
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 180 Issue 9 Pg. 6255-61 (May 01 2008) ISSN: 0022-1767 [Print] United States
• PMID: 18424748 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Extracellular Matrix Proteins
• Recombinant Proteins
• mindin

Topics

• Animals
• Dogs
• Extracellular Matrix Proteins (genetics, immunology, pharmacology)
• Immunity, Innate (genetics)
• Influenza A Virus, H1N1 Subtype (immunology)
• Influenza A Virus, H3N2 Subtype (immunology)
• Lung (immunology, virology)
• Macrophages (immunology, virology)
• Mice
• Mice, Knockout
• Nasal Cavity (immunology, virology)
• Orthomyxoviridae Infections (genetics, immunology)
• Recombinant Proteins (genetics, immunology, pharmacology)