Abstract |
We have investigated the relationship between the status of red blood cells (RBCs) and their susceptibility to toxin sticholysin II (StII) hemolytic activity; we have evaluated this effect in different RBC ensembles, comprising young and old cells, and in cells partially damaged by their pre-exposition to a free radical source. Upon action of StII, young cell populations are less prone to hemolysis than the whole population, while old cell populations and peroxyl-oxidized red cells are lysed faster than the whole population. Cell K(+) content was higher in young cells and lower in both senescent cells and in peroxyl-damaged cells relative to whole cell population. The relevance of cell K(+) content in St II-induced lysis was shown when external Na(+) was partially replaced by K(+); under this condition, RBC lysed faster in the presence of St II but no difference was observed among young cells, whole cells population and peroxyl-damaged cells; only old cells lysed faster that the whole population, response that can be due to an enhanced St II-induced pore formation as supported by evaluation of St II irreversible binding to RBC. It is concluded that this factor and the amount of intracellular K(+) are the dominant parameters that modulate the resistance of RBC to St II-induced lysis.
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Authors | Gloria Celedón, Gustavo González, Daniela Barrientos, Jose Pino, Fabiola Venegas, Eduardo A Lissi, Carmen Soto, Diana Martinez, Carlos Alvarez, María Eliana Lanio |
Journal | Toxicon : official journal of the International Society on Toxinology
(Toxicon)
Vol. 51
Issue 8
Pg. 1383-90
(Jun 15 2008)
ISSN: 0041-0101 [Print] England |
PMID | 18423792
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cnidarian Venoms
- Pore Forming Cytotoxic Proteins
- sticholysin II
- Potassium
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Topics |
- Animals
- Cell Separation
- Cnidarian Venoms
(pharmacology)
- Erythrocyte Aging
- Erythrocytes
(drug effects)
- Hemolysis
(drug effects)
- Osmotic Fragility
- Oxidative Stress
- Pore Forming Cytotoxic Proteins
(pharmacology)
- Potassium
(metabolism)
- Sea Anemones
(chemistry)
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