Emulsified isoflurane produces cardiac protection after ischemia-reperfusion injury in rabbits.

Abstract

BACKGROUND:

In this study, we examined the cardioprotective effects of parental emulsified isoflurane compared with inhaled isoflurane.

METHODS:

Thirty-two rabbits were subjected to 30 min of myocardial ischemia induced by temporary ligation of the left anterior descending coronary artery followed by 3 h of reperfusion. Before left anterior descending coronary artery occlusion, the rabbits were randomly allocated into one of four groups (eight for each group): group C, no ischemia preconditioning treatment; group IS, inhaled isoflurane 1.1% end-tidal; group EI, a continuous infusion of 8% emulsified isoflurane to an end-tidal concentration of 0.64%; and group IN, a continuous infusion of 30% Intralipid started 30 min. Treatments were started 30 min before ischemia followed by a 15 min washout period for isoflurane groups. Myocardial infarct volume, lactate dehydrogenase, and creatine kinase levels were measured and changes in mitochondrial ultrastructure assessed after 3 h myocardial reperfusion.

RESULTS:

Myocardial infarct size 3 h after reperfusion was lower in groups IS and EI compared with groups C and IN (20% +/- 8%, 18% +/- 8%, 39% +/- 6%, and 34% +/- 9%, respectively, P < 0.01). There were no differences in myocardial infarct size between groups IS and EI or between groups C and IN. Plasma lactate dehydrogenase and creatine kinase levels were lower in group IS (456 +/- 58 U/L and 1725 +/- 230 U/L) and group EI (451 +/- 54 U/L and 1686 +/- 444 U/L) 3 h after myocardial reperfusion compared with groups C (676 +/- 82 U/L and 2373 +/- 529 U/L; P < 0.01). Mitochondrial ultrastructure changes were less pronounced in groups IS and EI compared with group C.

CONCLUSIONS:

Our results indicate that, in rabbits, i.v. emulsified isoflurane provides similar myocardial protection against ischemia-reperfusion injury as inhaled isoflurane.

• Authors: Yan Rao, Yan-lin Wang, Wen-sheng Zhang, Jin Liu
• Journal: Anesthesia and analgesia (Anesth Analg) Vol. 106 Issue 5 Pg. 1353-9, table of contents (May 2008) ISSN: 1526-7598 [Electronic] United States
• PMID: 18420844 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anesthetics, Inhalation
• Fat Emulsions, Intravenous
• Protective Agents
• Isoflurane
• L-Lactate Dehydrogenase
• Creatine Kinase

Topics

• Administration, Inhalation
• Anesthetics, Inhalation (administration & dosage)
• Animals
• Coronary Vessels (surgery)
• Creatine Kinase (blood)
• Cytoprotection
• Disease Models, Animal
• Fat Emulsions, Intravenous (administration & dosage)
• Infusions, Intravenous
• Isoflurane (administration & dosage)
• L-Lactate Dehydrogenase (blood)
• Ligation
• Male
• Microscopy, Electron, Transmission
• Mitochondria, Heart (drug effects, ultrastructure)
• Myocardial Infarction (enzymology, etiology, pathology, prevention & control)
• Myocardial Ischemia (complications, drug therapy, enzymology, pathology)
• Myocardial Reperfusion Injury (enzymology, etiology, pathology, prevention & control)
• Myocardium (enzymology, pathology)
• Protective Agents (administration & dosage)
• Rabbits
• Time Factors