Abstract |
Glycyrrhiza uralensis (Leguminosae) has long been known as an antiinflammatory agent for gastric ulcers, arthritis, and rheumatism. The flavonoid glycyrol (GC) (10 microg/ml) isolated from G. uralensis dramatically inhibits phorbol ester (phorbol 12-myristate 13-acetate)-induced nuclear factor ( NF)-kappaB-dependent transcriptional activity, as determined by luciferase reporter activity in human kidney epithelial 293T cells. To investigate global gene expression profiling in cells by GC, we performed high-density oligonucleotide microarrays. Our microarray analyses showed that GC inhibited phorbol ester-induced NF-kappaB-dependent transcriptional activity in inflammatory-related gene expression. RT-PCR analysis, based on microarray data, showed that NF-kappaB-dependent genes (such as CCL2, CCL7, CD44, and HSPB8 in addition to NF-kappaB itself) were significantly downregulated by GC. Treatment with GC (10 microg/ml) inhibited I-kappaB degradation induced by phorbol 12-myristate 13-acetate. The microarray data also suggested that GC induces gene expression to p53-dependent apoptosis through endonuclease G, instead of CAD/DFF and AIF/PDCD8, as a downstream-apoptosis factor in human kidney epithelial 293T tumor cells, and induces oncogenes with a suppressor role as an added function.
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Authors | SungGa Lee, Hyun-Mee Oh, Won-Bong Lim, Eun-Ju Choi, Young-Na Park, Jeong-Ah Kim, Ji-Young Choi, Suk-Jin Hong, Hee-Kyun Oh, Jong-Keun Son, Seung-Ho Lee, Ok-Joon Kim, Hong-ran Choi, Chang-Duk Jun |
Journal | Anti-cancer drugs
(Anticancer Drugs)
Vol. 19
Issue 5
Pg. 503-15
(Jun 2008)
ISSN: 0959-4973 [Print] England |
PMID | 18418217
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Flavonoids
- NF-kappa B
- Tumor Suppressor Protein p53
- glycyrol
- Endodeoxyribonucleases
- endonuclease G
- Tetradecanoylphorbol Acetate
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Endodeoxyribonucleases
(genetics, metabolism)
- Flavonoids
(pharmacology)
- Gene Expression Profiling
- Glycyrrhiza uralensis
- Humans
- NF-kappa B
(biosynthesis)
- Oligonucleotide Array Sequence Analysis
- Oncogenes
(physiology)
- Reverse Transcriptase Polymerase Chain Reaction
- Tetradecanoylphorbol Acetate
(pharmacology)
- Tumor Suppressor Protein p53
(genetics, physiology)
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