HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Nordihydroguaiaretic acid restores expression of silenced E-cadherin gene in human breast cancer cell lines and xenografts.

Abstract
In our study we use nordihydroguaiaretic acid (NDGA), the naturally occurring lignan, to investigate whether it plays a role in the prevention and treatment of cancer by epigenetic modifications. The growth inhibitory effect of NDGA on human breast cancer cell lines was determined using the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay). It substantially inhibited the growth of human breast cancer cell lines SKBR3 and MDA-MB-435 with an estimated IC50 of 31.09+/-1.6 and 38.8+/-2.1 micromol/l respectively, after 4 days incubation with different NDGA concentrations. The in-vivo anticancer activity of NDGA was evaluated by calculating the tumor growth inhibition value. NDGA substantially inhibited the growth of human breast carcinoma cells in both animal and cell-based models. We also found that a single treatment with NDGA reactivates methylation-silenced E-cadherin gene in vitro and in vivo, suggesting an intriguing concept that lignans may act as natural effective epigenetic modifiers in the prevention and treatment of cancer.
AuthorsYi Cui, Cailing Lu, Aijun Kang, Liyong Liu, Sainan Tan, Daguang Sun, Jianguo Hu, Xu Ma
JournalAnti-cancer drugs (Anticancer Drugs) Vol. 19 Issue 5 Pg. 487-94 (Jun 2008) ISSN: 0959-4973 [Print] England
PMID18418215 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Cadherins
  • Masoprocol
Topics
  • Animals
  • Antineoplastic Agents, Phytogenic (pharmacology)
  • Breast Neoplasms
  • Cadherins (biosynthesis, genetics)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • DNA Methylation (drug effects)
  • Dose-Response Relationship, Drug
  • Epigenesis, Genetic
  • Female
  • Gene Silencing
  • Humans
  • Masoprocol (pharmacology)
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Transplantation, Heterologous

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: