Abstract |
In our study we use nordihydroguaiaretic acid (NDGA), the naturally occurring lignan, to investigate whether it plays a role in the prevention and treatment of cancer by epigenetic modifications. The growth inhibitory effect of NDGA on human breast cancer cell lines was determined using the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay). It substantially inhibited the growth of human breast cancer cell lines SKBR3 and MDA-MB-435 with an estimated IC50 of 31.09+/-1.6 and 38.8+/-2.1 micromol/l respectively, after 4 days incubation with different NDGA concentrations. The in-vivo anticancer activity of NDGA was evaluated by calculating the tumor growth inhibition value. NDGA substantially inhibited the growth of human breast carcinoma cells in both animal and cell-based models. We also found that a single treatment with NDGA reactivates methylation-silenced E-cadherin gene in vitro and in vivo, suggesting an intriguing concept that lignans may act as natural effective epigenetic modifiers in the prevention and treatment of cancer.
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Authors | Yi Cui, Cailing Lu, Aijun Kang, Liyong Liu, Sainan Tan, Daguang Sun, Jianguo Hu, Xu Ma |
Journal | Anti-cancer drugs
(Anticancer Drugs)
Vol. 19
Issue 5
Pg. 487-94
(Jun 2008)
ISSN: 0959-4973 [Print] England |
PMID | 18418215
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Cadherins
- Masoprocol
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Topics |
- Animals
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Breast Neoplasms
- Cadherins
(biosynthesis, genetics)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- DNA Methylation
(drug effects)
- Dose-Response Relationship, Drug
- Epigenesis, Genetic
- Female
- Gene Silencing
- Humans
- Masoprocol
(pharmacology)
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Transplantation, Heterologous
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