Abstract | BACKGROUND: The display of binding ligands, such as recombinant antibody fragments, on the surface of filamentous phage makes it possible to specifically attach these phage particles to target cells. After uptake of the phage, their internal single-stranded DNA is processed by the host cell, which allows transient expression of an encoded eukaryotic gene cassette. This opens the possibility to use bacteriophage as vectors for targeted gene therapy, although the transduction efficiency is very low. RESULTS: CONCLUSION: We can conclude from the results that the postulated multivalency of the anti-CD30-pVIII displaying bacteriophage combined with disseminated display of the anti-CD30 scFv on the whole particle surface is responsible for the improved gene transfer rate. These results mark an important step towards the use of phage particles as a cheap and safe gene transfer vehicle for the gene delivery of the desired target cells via their specific surface receptors.
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Authors | Yoon-Suk A Chung, Katja Sabel, Martin Krönke, Alexander Klimka |
Journal | BMC molecular biology
(BMC Mol Biol)
Vol. 9
Pg. 37
(Apr 16 2008)
ISSN: 1471-2199 [Electronic] England |
PMID | 18416816
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Capsid Proteins
- Immunoglobulin Variable Region
- Ki-1 Antigen
- Peptide Library
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Topics |
- Bacteriophage M13
(genetics, metabolism)
- Capsid Proteins
(metabolism)
- Cell Aggregation
- Cell Line, Tumor
- Gene Transfer Techniques
- Genetic Vectors
- Hodgkin Disease
(metabolism, pathology)
- Humans
- Immunoglobulin Variable Region
(genetics, metabolism)
- Ki-1 Antigen
(immunology)
- Peptide Library
- Protein Binding
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