Abstract | OBJECTIVE: METHODS: Rats were subjected to a partial ligation of the sciatic nerve, a model of neuropathic pain, and were treated either systemically or locally (hindpaw) with the H(3)/H(4) receptor inverse agonist thioperamide, the specific H(4) receptor antagonist JNJ 7777120, or the H(4) receptor agonist VUF 8430. Measurements of mechanical hyperalgesia were carried out by Randall-Selitto test for 1-3 weeks, and sciatic nerve tissues were analyzed for numbers of intact mast cells by histology at 9 h after surgery. RESULTS: Rats treated with thioperamide or JNJ 7777120 showed significantly enhanced mechanical hyperalgesia after partial ligation of the sciatic nerve. The number of intact mast cells in the injured nerve of these rats was higher than in control rats suggesting reduced mast cell degranulation, but was still significantly lower than in intact nerves. Rats treated with VUF 8430 showed significantly reduced mechanical hyperalgesia. CONCLUSION:
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Authors | Fiona M Smith, Hila Haskelberg, David J Tracey, Gila Moalem-Taylor |
Journal | Neuroimmunomodulation
(Neuroimmunomodulation)
Vol. 14
Issue 6
Pg. 317-25
( 2007)
ISSN: 1423-0216 [Electronic] Switzerland |
PMID | 18401194
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | (c) 2008 S. Karger AG, Basel. |
Chemical References |
- Guanidines
- Histamine Antagonists
- Hrh4 protein, rat
- Indoles
- Piperazines
- Piperidines
- Receptors, G-Protein-Coupled
- Receptors, Histamine
- Receptors, Histamine H3
- Receptors, Histamine H4
- S-(2-guanidylethyl)isothiourea
- 1-((5-chloro-1H-indol-2-yl)carbonyl)-4-methylpiperazine
- Thiourea
- thioperamide
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Topics |
- Animals
- Guanidines
(pharmacology)
- Histamine Antagonists
(pharmacology)
- Hyperalgesia
(physiopathology)
- Indoles
(pharmacology)
- Ligation
- Male
- Mast Cells
(metabolism)
- Neuralgia
(physiopathology)
- Pain Threshold
(drug effects, physiology)
- Piperazines
(pharmacology)
- Piperidines
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Rats, Wistar
- Receptors, G-Protein-Coupled
(drug effects, metabolism)
- Receptors, Histamine
(drug effects, metabolism)
- Receptors, Histamine H3
(drug effects, metabolism)
- Receptors, Histamine H4
- Sciatic Nerve
(cytology, injuries)
- Thiourea
(analogs & derivatives, pharmacology)
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