Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding.
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| Abstract |
A new metabolite profiling approach combined with an ultrarapid sample preparation procedure was used to study the temporal and spatial dynamics of the wound-induced accumulation of jasmonic acid (JA) and its oxygenated derivatives in Arabidopsis thaliana. In addition to well known jasmonates, including hydroxyjasmonates (HOJAs), jasmonoyl-isoleucine (JA-Ile), and its 12-hydroxy derivative (12-HOJA-Ile), a new wound-induced dicarboxyjasmonate, 12-carboxyjasmonoyl-l-isoleucine (12-HOOCJA-Ile) was discovered. HOJAs and 12-HOOCJA-Ile were enriched in the midveins of wounded leaves, strongly differentiating them from the other jasmonate metabolites studied. The polarity of these oxylipins at physiological pH correlated with their appearance in midveins. When the time points of accumulation of different jasmonates were determined, JA levels were found to increase within 2-5 min of wounding. Remarkably, these changes occurred throughout the plant and were not restricted to wounded leaves. The speed of the stimulus leading to JA accumulation in leaves distal to a wound is at least 3 cm/min. The data give new insights into the spatial and temporal accumulation of jasmonates and have implications in the understanding of long-distance wound signaling in plants.
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| Authors | Gaetan Glauser, Elia Grata, Lucie Dubugnon, Serge Rudaz, Edward E Farmer, Jean-Luc Wolfender |
| Journal | The Journal of biological chemistry (J Biol Chem) Vol. 283 Issue 24 Pg. 16400-7 (Jun 13 2008) ISSN: 0021-9258 [Print] United States |
| PMID | 18400744 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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