A potent tyrosinase activator from Radix Polygoni multiflori and its melanogenesis stimulatory effect in B16 melanoma cells.

Tyrosinase is a key enzyme in melanin biosynthesis. Activators of tyrosinase with stimulatory effects on melanogenesis are beneficial for the treatment of hypopigmentation diseases. In the present study, mushroom tyrosinase activity assay was performed to screen tyrosinase activators from traditional Chinese herbs. Four components from Radix Polygoni multiflori were tested. The most active compound, 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG), was found to be a significant tyrosinase activator. The maximal activation was 126% at a concentration of 75.0 microg/mL. The three anthraquinones slightly activated tyrosinase with effects in the range 7-31%. All the compounds were tested in B16 melanoma cells, the anthraquinones were found to inhibit cell proliferation at a concentration of 0.1-2.5 microg/mL, and THSG was found to be non-cytotoxic at a concentration of 0.1-12.5 microg/mL. THSG significantly increased the activity of murine tyrosinase and stimulated melanin biosynthesis in B16 melanoma cells. In conclusion, THSG is a potent tyrosinase activator and stimulator of melanogenesis with potential for the treatment of hypopigmentation disease.
AuthorsShuyu Guan, Weiwei Su, Ning Wang, Peibo Li, Yonggang Wang
JournalPhytotherapy research : PTR (Phytother Res) Vol. 22 Issue 5 Pg. 660-3 (May 2008) ISSN: 1099-1573 [Electronic] England
PMID18389468 (Publication Type: Journal Article)
Chemical References
  • 2,3,5,4'-tetrahydroxystilbene-2-O-glucoside
  • Anthraquinones
  • Glucosides
  • Melanins
  • Plant Extracts
  • Stilbenes
  • Monophenol Monooxygenase
  • Animals
  • Anthraquinones (pharmacology)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Dose-Response Relationship, Drug
  • Enzyme Activation (drug effects)
  • Glucosides (pharmacology)
  • Melanins (metabolism)
  • Melanoma, Experimental (metabolism, pathology)
  • Mice
  • Monophenol Monooxygenase (metabolism)
  • Plant Extracts (chemistry, pharmacology)
  • Polygonum (chemistry)
  • Stilbenes (pharmacology)

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