Abstract | OBJECTIVE: METHODS: Female Sprague-Dawley rats received a single intraperitoneal injection of CYP (200 mg/kg) or saline. After the CYP injection, eviprostat (9, 18 or 54 mg/kg per day) or a vehicle was orally given twice each day. Four days after the CYP injection, bladder function was evaluated by cystometrograms under urethane anesthesia. In a separate group, bladder inflammation was compared between the eviprostat- or vehicle-treated animals. Furthermore, the effects of eviprostat on carbachol-induced muscle contraction were evaluated by an in vitro experiment. RESULTS: The intercontraction interval (ICI) significantly decreased in the CYP-injected rats in comparison to the saline-injected rats. In the CYP-injected group, 18 and 54 mg/kg per day of eviprostat treatment significantly increased the ICI, but did not change the maximum voiding pressure in comparison to the vehicle treatment. In the saline-injected group, no significant changes of any parameters in the cystometrograms were observed between the eviprostat- and vehicle-treated groups. CYP-induced bladder inflammation tended to be lower in the eviprostat-treated group in comparison to the vehicle-treated group. An in vitro experiment revealed that eviprostat failed to inhibit carbachol-induced muscle contraction. CONCLUSION:
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Authors | Mizuki Kobayashi, Masayoshi Nomura, Hisae Nishii, Seiji Matsumoto, Naohiro Fujimoto, Tetsuro Matsumoto |
Journal | International journal of urology : official journal of the Japanese Urological Association
(Int J Urol)
Vol. 15
Issue 4
Pg. 356-60
(Apr 2008)
ISSN: 1442-2042 [Electronic] Australia |
PMID | 18380828
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Alkylating
- Cholinergic Agonists
- Drug Combinations
- Plant Extracts
- Ethamsylate
- eviprostat
- Cyclophosphamide
- Carbachol
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Topics |
- Animals
- Antineoplastic Agents, Alkylating
- Carbachol
- Cholinergic Agonists
- Cyclophosphamide
- Cystitis
(chemically induced, drug therapy, pathology)
- Drug Combinations
- Ethamsylate
(pharmacology, therapeutic use)
- Female
- Muscle Contraction
(drug effects)
- Organ Size
(drug effects)
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Urinary Bladder
(drug effects, pathology)
- Urinary Bladder, Overactive
(chemically induced, drug therapy, pathology)
- Urination
(drug effects)
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