Cytotoxic constituents from Podocarpus fasciculus.

Abstract

A new diterpene, 16-hydroxy communic acid (1), along with thirty one known compounds including five norditerpenes (2-6), twenty two flavonoids containing four biflavonoids (7-10), nine monoflavonoids (11-19) and nine flavanoid glycosides (20-28), as well as four phenolic constituents (29-32) were isolated from the 95% ethanolic extract of Podocarpus fasciculus. The structure of 1 was elucidated using spectral methods. Of these isolates, nagilactone C (2) showed the most significant inhibitory effects against DLD cells (human colon carcinoma) (ED(50)=2.57 microg/ml) and compounds 7, 8, 10, 11, and 12 had moderate cytotoxic activity against human KB (human oral epithelium carcinoma), Hela (human cervical carcinoma), Hepa (human hepatoma), DLD (colon carcinoma), and A-549 (human lung carcinoma) tumor cell lines. Preliminary structure-activity relationship studies of the isolated diterpenoids and biflavonoids are discussed.

• Authors: Yu-Jen Kuo, Shy-Yuan Hwang, Ming-Der Wu, Chia-Ching Liao, Yu-Han Liang, Yao-Haur Kuo, Hsiu-O Ho
• Journal: Chemical & pharmaceutical bulletin (Chem Pharm Bull (Tokyo)) Vol. 56 Issue 4 Pg. 585-8 (Apr 2008) ISSN: 0009-2363 [Print] Japan
• PMID: 18379113 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 16-hydroxycommunic acid
• Antineoplastic Agents, Phytogenic
• Diterpenes
• Indicators and Reagents
• Tetrazolium Salts
• Thiazoles
• thiazolyl blue

Topics

• Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
• Carbohydrate Conformation
• Cell Line, Tumor
• Chromatography, High Pressure Liquid
• Diterpenes (chemistry, pharmacology)
• Drug Screening Assays, Antitumor
• Humans
• Indicators and Reagents
• Spectrometry, Mass, Electrospray Ionization
• Spectrophotometry, Infrared
• Spectrophotometry, Ultraviolet
• Structure-Activity Relationship
• Tetrazolium Salts
• Thiazoles
• Tracheophyta (chemistry)