Abstract | BACKGROUND: OBJECTIVES: In the present study we investigate the prevalence of the different HBV genotypes in Morocco since no previous careful study has been attempted. METHODS: Epidemiological data from 91 chronically infected patients (45 women and 46 men) were collected prospectively. Sera were tested for anti-HBc IgG, HBeAg, anti-HBe antibody and liver enzymes. Restriction Fragment Length Polymorphism (RFLP) analysis was confirmed by subsequent sequencing of the pre-S and S region of the viral genome in order to determine which HBV genotypes were prevalent among Moroccan patients. RESULTS: The mean age was 41+/-12.4 years. Ten patients (11%) were positive for hepatitis B e antigen ( HBeAg) and 81 (89%) were positive for anti-HBe antibodies. By the RLFP method, genotype D, pattern D2, was found in the 77 cases where HBV was successfully amplified. Phylogenetic analysis based on pre-S/S sequences revealed that genotype D in Morocco differed from others D strains subgenotypes (D1, D2, D3 and D4). In addition, the pre-core mutant defined as HBeAg-negative/anti-HBe-positive and HBV DNA positive was detected in 86% of cases. CONCLUSIONS: Our results clearly show that genotype D and pre-core mutant are highly prevalent in Morocco.
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Authors | Sayeh Ezzikouri, Isabelle Chemin, Abdelaziz Chafik, Lahsen Wakrim, Jalal Nourlil, Abdelouhad El Malki, Agnès Marchio, Anne Dejean, Mohammed Hassar, Christian Trepo, Pascal Pineau, Soumaya Benjelloun |
Journal | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
(Infect Genet Evol)
Vol. 8
Issue 3
Pg. 306-12
(May 2008)
ISSN: 1567-1348 [Print] Netherlands |
PMID | 18372221
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- DNA, Viral
(analysis)
- Genes, Viral
- Genotype
- Hepatitis B virus
(genetics)
- Hepatitis B, Chronic
(genetics, virology)
- Heterozygote
- Humans
- Morocco
- Phylogeny
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