Escherichia coli Nissle 1917 facilitates tumor detection by positron emission tomography and optical imaging.

Bacteria-based tumor-targeted therapy is a modality of growing interest in anticancer strategies. Imaging bacteria specifically targeting and replicating within tumors using radiotracer techniques and optical imaging can provide confirmation of successful colonization of malignant tissue.
The uptake of radiolabeled pyrimidine nucleoside analogues and [18F]FDG by Escherichia coli Nissle 1917 (EcN) was assessed both in vitro and in vivo. The targeting of EcN to 4T1 breast tumors was monitored by positron emission tomography (PET) and optical imaging. The accumulation of radiotracer in the tumors was correlated with the number of bacteria. Optical imaging based on bioluminescence was done using EcN bacteria that encode luciferase genes under the control of an l-arabinose-inducible P(BAD) promoter system.
We showed that EcN can be detected using radiolabeled pyrimidine nucleoside analogues, [18F]FDG and PET. Importantly, this imaging paradigm does not require transformation of the bacterium with a reporter gene. Imaging with [18F]FDG provided lower contrast than [18F]FEAU due to high FDG accumulation in control (nontreated) tumors and surrounding tissues. A linear correlation was shown between the number of viable bacteria in tumors and the accumulation of [18F]FEAU, but not [18F]FDG. The presence of EcN was also confirmed by bioluminescence imaging.
EcN can be imaged by PET, based on the expression of endogenous E. coli thymidine kinase, and this imaging paradigm could be translated to patient studies for the detection of solid tumors. Bioluminescence imaging provides a low-cost alternative to PET imaging in small animals.
AuthorsPeter Brader, Jochen Stritzker, Christopher C Riedl, Pat Zanzonico, Shangde Cai, Eva M Burnazi, E Rashid Ghani, Hedvig Hricak, Aladar A Szalay, Yuman Fong, Ronald Blasberg
JournalClinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 14 Issue 8 Pg. 2295-302 (Apr 15 2008) ISSN: 1078-0432 [Print] United States
PMID18369089 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Fluorodeoxyglucose F18
  • Arabinofuranosyluracil
  • 2'-fluoro-5-ethylarabinosyluracil
  • Thymidine Kinase
  • Animals
  • Arabinofuranosyluracil (analogs & derivatives, pharmacokinetics)
  • Cell Line, Tumor
  • Escherichia coli
  • Fluorodeoxyglucose F18 (pharmacokinetics)
  • Herpesvirus 1, Human (enzymology)
  • Mice
  • Neoplasms, Experimental (microbiology, radionuclide imaging)
  • Positron-Emission Tomography
  • Probiotics (pharmacology)
  • Thymidine Kinase (metabolism)
  • Tissue Distribution

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