The effect of an anti-inflammatory pentapeptide produced by Entamoeba histolytica on gene expression in the U-937 monocytic cell line.

Abstract

OBJECTIVE:

Evaluate the Monocyte Locomotion Inhibitory Factor (MLIF) effect upon the expression of genes encoding human cytokines, receptors and related factors in the human cell line U-937. MLIF (Met-Gln-Cys-Asn-Ser) is an anti-inflammatory pentapeptide produced by Entamoeba histolytica that inhibits many human monocyte functions.

MATERIAL AND METHODS:

U-937 cell line cultured (24 hrs/RPMI). RNA extracted by Trizol method. 385 genes were analyzed on microarray membranes, complement by real-time RT-PCR and protein expression of some affected genes.

RESULTS:

MLIF had a preferentially inhibitory effect on gene expression; four genes were over-expressed and 13 underexpressed in MILF vs. simple medium - constitutive expression. Three genes are over-expressed and 19 under-expressed in MLIF/PMA vs. PMA - induced expression.

CONCLUSIONS:

Many modified genes are products regulated by the Nuclear Factor-kappaB and Mitogen Activated Protein Kinase pathways, suggesting MLIF involvement with these two major pathways for the modulation of the inflammation and immune responses.

• Authors: R Silva-García, I Estrada-García, R Ramos-Payán, A Torres-Salazar, M E Morales-Martínez, D Arenas-Aranda, J A Giménez-Scherer, F Blanco-Favela, M G Rico-Rosillo
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 57 Issue 4 Pg. 145-50 (Apr 2008) ISSN: 1023-3830 [Print] Switzerland
• PMID: 18368290 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• NF-kappa B
• Oligopeptides
• monocyte locomotion inhibitory factor
• Mitogen-Activated Protein Kinase Kinases

Topics

• Animals
• Cytokines (genetics, metabolism)
• Entamoeba histolytica (metabolism)
• Gene Expression (drug effects)
• Gene Expression Profiling
• Humans
• Inflammation (metabolism)
• Mitogen-Activated Protein Kinase Kinases (metabolism)
• Models, Biological
• Monocytes (cytology, metabolism)
• NF-kappa B (metabolism)
• Oligonucleotide Array Sequence Analysis
• Oligopeptides (metabolism, pharmacology)
• U937 Cells